ATP9A Antibody

Datasheet

Code	CSB-PA002422LA01HU
Size	$600
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Image	IHC image of CSB-PA002422LA01HU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunofluorescence staining of A549 cell with CSB-PA002422LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate All lanes: ATP9A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 118 kDa Observed band size: 118 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP9A Polyclonal antibody

Uniprot No.

O75110

Target Names

ATP9A

Alternative Names

ATP 9A antibody; Atp9a antibody; ATP9A_HUMAN antibody; ATPase class II type 9A antibody; ATPase IIA antibody; ATPase type IV phospholipid transporting (P type) antibody; ATPase type IV phospholipid transporting (P type)(putative) antibody; ATPIIA antibody; KIAA0611 antibody; Phospholipid transporting ATPase IIA antibody; Probable phospholipid transporting ATPase IIA antibody; Probable phospholipid-transporting ATPase IIA antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Probable phospholipid-transporting ATPase IIA protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP9A Antibody (Product code: CSB-PA002422LA01HU) is Non-conjugated. For ATP9A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002422LB01HU	ATP9A Antibody, HRP conjugated	ELISA
FITC	CSB-PA002422LC01HU	ATP9A Antibody, FITC conjugated
Biotin	CSB-PA002422LD01HU	ATP9A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:50-1:200
IF	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling and endosome to trans-Golgi network retrograde transport. In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation. Appears to be implicated in intercellular communication by negatively regulating the release of exosomes. The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved.

Gene References into Functions

the predominant P4 ATPases in pure pancreatic beta cells and human and rat pancreatic islets were ATP8B1, ATP8B2, and ATP9A. ATP8B1 and CDC50A were highly concentrated in ISG PMID: 26240149
Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826

Subcellular Location

Early endosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Protein Families

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IV subfamily

Database Links

HGNC: 13540

OMIM: 609126

KEGG: hsa:10079

STRING: 9606.ENSP00000342481

UniGene: Hs.649234

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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