COX5B Antibody

Code CSB-PA00404A0Rb
Size US$166
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  • Western blot
    All lanes: COX5B antibody at 2µg/ml + EC109 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/15000 dilution
    Predicted band size: 14 kDa
    Observed band size: 14, 65 kDa

  • Immunofluorescent analysis of MCF-7 cells using CSB-PA00404A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) COX5B Polyclonal antibody
Uniprot No.
Target Names
COX5B
Alternative Names
COX 5B antibody; COX5B antibody; COX5B_HUMAN antibody; COXVB antibody; Cytochrome c oxidase polypeptide Vb antibody; Cytochrome c oxidase polypeptide VB mitochondrial antibody; Cytochrome c oxidase subunit 5B antibody; Cytochrome c oxidase subunit 5B mitochondrial antibody; Cytochrome c oxidase subunit Vb antibody; mitochondrial antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Cytochrome c oxidase subunit 5B, mitochondrial protein (32-129AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The COX5B Antibody (Product code: CSB-PA00404A0Rb) is Non-conjugated. For COX5B Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA00404B0Rb COX5B Antibody, HRP conjugated ELISA
FITC CSB-PA00404C0Rb COX5B Antibody, FITC conjugated
Biotin CSB-PA00404D0Rb COX5B Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Gene References into Functions
  1. Our results suggest that COX5B protein level might be associated with tumor size. COX5B overexpression indicated a worse DFS (p < 0.05) in breast cancer. PMID: 28592145
  2. These experiments suggest that the FAD group of cytochrome b5 reductase increase its solvent exposition upon complex formation promoting an efficient electron transfer between the proteins. PMID: 29111436
  3. silence of COX5B leads to metabolic disorders, such as increased glucose uptake and decreased lactate secretion PMID: 26506233
  4. Transfected the A549 cells with COX Vb small interfering or shRNA and observed a significant reduction of their COX activity, oxygen consumption, ATP and ability to grow in soft agar and as poorly differentiated tumors in athymic mice. PMID: 22917272
  5. Data show that Bcl-2 expression positively influences the targeting of nuclear-encoded COX Va and Vb to the mitochondria of cancer cells. PMID: 19834492

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Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Protein Families
Cytochrome c oxidase subunit 5B family
Database Links

HGNC: 2269

OMIM: 123866

KEGG: hsa:1329

STRING: 9606.ENSP00000258424

UniGene: Hs.1342

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