DAP Antibody

Datasheet

Code	CSB-PA01034A0Rb
Size	US$166
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Image	IHC image of CSB-PA01034A0Rb diluted at 1:150 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA01034A0Rb diluted at 1:150 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DAP Polyclonal antibody

Uniprot No.

P51397

Target Names

DAP

Alternative Names

DAP 1 antibody; Dap antibody; DAP-1 antibody; DAP1_HUMAN antibody; Death associated protein 1 antibody; Death associated protein antibody; Death-associated protein 1 antibody; MGC99796 antibody; OTTHUMP00000161670 antibody; OTTHUMP00000221331 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Death-associated protein 1 protein (2-102AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DAP Antibody (Product code: CSB-PA01034A0Rb) is Non-conjugated. For DAP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA01034B0Rb	DAP Antibody, HRP conjugated	ELISA
FITC	CSB-PA01034C0Rb	DAP Antibody, FITC conjugated
Biotin	CSB-PA01034D0Rb	DAP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death.

Gene References into Functions

DAP1 may have an important role in cell adhesion, migration and growth in the context of breast cancer and has significant associations with the apoptosis pathway. PMID: 25530065
DAP1 was found to be correlated with disease progression and long-term survival of the colorectal patients. DAP1 is also a pivotal regulator of the growth and apoptosis and cellular response to chemotherapy agents. PMID: 24270644
This study demonstrates an inverse association between DAP1 mRNA levels and tumour stage and clinical outcome in breast cancer PMID: 22798505
Mapping of the phosphorylation sites and analysis of phosphorylation mutants indicated that DAP1 is functionally silenced in growing cells through mTOR-dependent phosphorylations on Ser3 and Ser51 PMID: 20537536

Database Links

HGNC: 2672

OMIM: 600954

KEGG: hsa:1611

STRING: 9606.ENSP00000230895

UniGene: Hs.75189

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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