E2F6 Antibody

Code CSB-PA002238
Size US$100
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  • Western Blot analysis of HepG2 cells using E2F-6 Polyclonal Antibody
  • Western Blot analysis of NIH-3T3 cells using E2F-6 Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
E2F6
Alternative Names
E2F 6 antibody; E2F binding site modulating activity protein antibody; E2F transcription factor 6 antibody; E2F transcription factor 6 isoform 1 antibody; E2F-6 antibody; E2F6 antibody; E2F6_HUMAN antibody; EMA antibody; MGC111545 antibody; Transcription factor E2F6 antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthesized peptide derived from the Internal region of Human E2F-6.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. Represses expression of some meiosis-specific genes, including SLC25A31/ANT4. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
Gene References into Functions
  1. This analysis showed a relative increase in the expression of E2F6 in gastric adenocarcinoma with no lymph node metastasis (chi (2), P = 0.04 and OR, P = 0.08), while overexpression of RhoA and SMUG1 was found more often in the diffuse subtype of gastric adenocarcinoma as compared to the intestinal subtype. PMID: 27909884
  2. After replication stress, the checkpoint kinase Chk1 phosphorylates E2F6, leading to its dissociation from promoters. This promotes E2F-dependent transcription, which mediates cell survival by preventing DNA damage and cell death PMID: 23954429
  3. findings indicate an inhibitory role of E2F6 in the regulation of IL-13 and allergy PMID: 22981205
  4. E2F6 may recruit BRG1 in transcriptional regulation of genes important for G1/S phase transition of the cell cycle. PMID: 23082233
  5. data suggest that E2F- and Myc-responsive genes are coregulated by E2F6 complex in quiescent cells PMID: 12004135
  6. results suggest that E2F6 represses transcription of the brca1, ctip, art27, hp1alpha, and the rbap48 genes and depletion of E2F6 resulted in the recruitment of E2F1 to the target promoters PMID: 12909625
  7. contains nine exons distributed along 20.4kbp of genomic DNA on chromosome 2 leading to the transcription of six alternatively spliced E2F6 mRNAs that encode four different E2F6 proteins PMID: 15081404
  8. E2F6 does not contain the domains required for modulation of squamous differentiation PMID: 15474455
  9. showed that E2F6, DP1, EPC1, EZH2, and Sin3B co-elute, suggesting the identification of a novel E2F6 complex that exists in vivo in both normal and transformed human cell lines PMID: 15536069
  10. functions as a repressor of E2F-dependent transcription during S phase PMID: 15574595
  11. PHC3 and E2F6 showed nuclear colocalization in a punctate pattern which was not seen in proliferating cells suggesting that PHC3 may be part of an E2F6-polycomb complex that has been shown to occupy and silence target promoters in G(0) PMID: 17001316
  12. E2F-6 inhibited Apaf-1 upregulation by competing with E2F-1 for promoter binding. It enhanced the clonogenic growth of granulocyte, erythroid, macrophage, & megakaryocyte cells.It may prevent hematopoietic progenitor cell loss during proliferation. PMID: 17600109
  13. E2F6 binding sites are located within 2 kb of a transcription start site, in both normal and tumor cells PMID: 17908821

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Subcellular Location
Nucleus.
Protein Families
E2F/DP family
Tissue Specificity
Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
Database Links

HGNC: 3120

OMIM: 602944

KEGG: hsa:1876

STRING: 9606.ENSP00000370936

UniGene: Hs.603093

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