EIF4A2 Antibody

Datasheet

Code	CSB-PA00384A0Rb
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: EIF4A2 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00384A0Rb at dilution of 1:100 Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA00384A0Rb at dilution of 1:100 Immunofluorescence staining of A549 cells with CSB-PA00384A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EIF4A2 Polyclonal antibody

Uniprot No.

Q14240

Target Names

EIF4A2

Alternative Names

ATP dependent RNA helicase eIF4A 2 antibody; ATP-dependent RNA helicase eIF4A-2 antibody; BM-010 antibody; DDX2B antibody; eIF 4A II antibody; eIF-4A-II antibody; EIF4A antibody; eIF4A II antibody; eIF4A-II antibody; EIF4A2 antibody; EIF4F antibody; Eukaryotic initiation factor 4A II antibody; Eukaryotic initiation factor 4A-II antibody; eukaryotic translation initiation factor 4A isoform 2 antibody; Eukaryotic translation initiation factor 4A2 antibody; IF4A2_HUMAN antibody; N-terminally processed antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Eukaryotic initiation factor 4A-II protein (1-407AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EIF4A2 Antibody (Product code: CSB-PA00384A0Rb) is Non-conjugated. For EIF4A2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA00384B0Rb	EIF4A2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA00384C0Rb	EIF4A2 Antibody, FITC conjugated
Biotin	CSB-PA00384D0Rb	EIF4A2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Gene References into Functions

eIF4A2 is recruited to stress granules, suggesting sumoylation of eIF4A2 correlates with its recruitment to stress granules. PMID: 27160682
expression is an independent prognostic factor for patients with non-small-cell lung cancer for both overall survival and disease-free survival PMID: 23867391
These data support a linear model for miRNA-mediated gene regulation in which translational repression via eIF4A2 is required first, followed by mRNA destabilization. PMID: 23559250
interacted with NS5B protein, and the two proteins were shown to be partially colocalized in the perinuclear region PMID: 11922617
Single nucleotide polymorphisms in two relevant candidate genes for glucose homeostasis, kininogen (KNG1), and eukaryotic translation initiation factor 4alpha2 (EIF4A2) linked to type 2 diabetes. PMID: 16567544
subcellular distribution of eIF4F components may potentiate the complex assembly PMID: 18250159
A feedforward loop involving c-Myc and eIF4F that serves to link transcription and translation and that could contribute to the effects of c-Myc on cell proliferation and neoplastic growth. PMID: 18593934
This protein has been found differentially expressed in the temporal lobe from patients with schizophrenia. PMID: 19034380

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Protein Families

DEAD box helicase family, eIF4A subfamily

Database Links

HGNC: 3284

OMIM: 601102

KEGG: hsa:1974

STRING: 9606.ENSP00000326381

UniGene: Hs.518475

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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