ERVFRD-1 Antibody

Datasheet

Code	CSB-PA352689ESR2HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: ERVFRD-1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa Immunofluorescence staining of Hela cell with CSB-PA352689LA01HU at 1:160, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ERVFRD-1 Polyclonal antibody

Uniprot No.

P60508

Target Names

ERVFRD-1

Alternative Names

EFRD1_HUMAN antibody; Endogenous retrovirus group FRD member 1 antibody; Envelope polyprotein antibody; ERVFRD-1 antibody; FLJ41944 antibody; FLJ90611 antibody; GLLL6191 antibody; HERV FRD 6p24.1 provirus ancestral Env polyprotein antibody; HERV FRD antibody; HERV FRD provirus ancestral Env polyprotein antibody; HERV-FRD antibody; HERVFRD antibody; HERVFRD provirus ancestral Env polyprotein antibody; MGC87585 antibody; SU antibody; Syncytin 2 antibody; Syncytin B antibody; Syncytin-2 antibody; TM antibody; Transmembrane protein antibody; UNQ6191 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Syncytin-2 protein (16-250AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The rabbit was immunized with recombinant human Syncytin-2 protein (16-250aa) to produce the ERVFRD-1 polyclonal antibody, which is an IgG isoform and exhibits a liquid form. This ERVFRD-1 antibody underwent antigen affinity purification. This ERVFRD-1 antibody shows reactivity with the human ERVFRD-1 protein.

The specificity of this ERVFRD-1 antibody has been validated in ELISA, WB, and IHC. The ERVFRD-1 polyclonal antibody can be used to detect and study the expression of the ERVFRD-1 protein as well as to visualize the distribution of the ERVFRD-1 protein. ERVFRD-1 is a critical protein for placental development and the healthy development of the fetus during pregnancy.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. The interaction with MFSD2A is apparently important for this process.; Endogenous envelope proteins may have kept, lost or modified their original function during evolution but this one can still make pseudotypes with MLV, HIV-1 or SIV-1 virions and confer infectivity. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein mediates receptor recognition, while the transmembrane protein anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.

Gene References into Functions

genetic predisposition in ERVFRDE-1 may be associated with an increased risk of preeclampsia. This polymorphism is possibly involved in the regulation of syncytin-2 expression in preeclamptic placenta. PMID: 29750965
N-glycans at residues 133, 312, 332 and 443 of syncytin-2 are required for optimal fusion induction, and that single-nucleotide polymorphism C46R, N118S, T367M, R417H, V483I and T522M can alter the fusogenic function of syncytin-2. PMID: 26853155
Decreased syncytin-2 and MFSD2 proteins in gestational diabetic placentas might cause abnormal syncytiotrophoblast formation and possibly be involved in the pathology PMID: 26875564
ERVWE1, ERVFRDE1 and ERV3 transcription was down-regulated in hydatidiform moles and gestational trophoblastic neoplasia. PMID: 26992684
These results thereby demonstrate that induced expression of Syncytin-2 is highly dependent on the interaction of bZIP-containing transcription factors to a CRE/AP-1 motif and that this element is important for the regulation of Syncytin-2 expression PMID: 25781974
MFSD2a, the Syncytin-2 receptor, is important for trophoblast fusion. PMID: 23177091
results further highlighted the existence of a correlation between the extent of the decrease in the expression levels of both syncytins 1 and 2 fusogenic proteins and the degree of severity of preeclampsia symptoms PMID: 21493955
Analysis of non-spliced ERVFRDE1 mRNAs and env mRNAs detected efficient splicing of endogenously expressed RNAs in trophoblastic but not in non-placental cells. PMID: 21771862
The crystal structure of a central fragment of syncytin 2 "fossil" ectodomain is reported, allowing a remarkable superposition with the structures of the corresponding domains of present-day infectious retroviruses PMID: 16140326
immunolocalized only in the villous trophoblast of the chorionic villi, at the level of cytotrophoblastic cells PMID: 16714059
findings show that in both humans and mice, one of the two syncytins (human syncytin-2 and mouse syncytin-B) is immunosuppressive and, rather unexpectedly, the other (human syncytin-1 and mouse syncytin-A) is not PMID: 18077339
Syncytin 2 expression illustrates the abnormal trophoblast differentiation observed in placenta of fetal T21-affected pregnancies. PMID: 18215254
expression decreased in preeclamptic placentas; may function as a second fusogenic protein for placental cell fusion PMID: 18650494
These results highlight the importance of Syncytin-2 in BeWo and primary human trophoblast cell fusion. PMID: 19616006

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Subcellular Location

Virion.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Transmembrane protein]: Cell membrane; Single-pass membrane protein.

Protein Families

Gamma type-C retroviral envelope protein family, HERV class-I FRD env subfamily

Tissue Specificity

Expressed at higher level in placenta. Expressed at lower level in adrenal, bone marrow, brain, breast, colon, kidney, lung, ovary, peripheral blood lymphocytes, prostate, skin, spleen, testis, thymus, thyroid, trachea.

Database Links

HGNC: 33823

OMIM: 610524

KEGG: hsa:405754

STRING: 9606.ENSP00000420174

UniGene: Hs.631996

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