FUNDC1 Antibody

Datasheet

Code	CSB-PA816886LA01HU
Size	$600
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate All lanes: FUNDC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Western Blot Positive WB detected in: Mouse Brain tissue lysate, Mouse Liver tissue lysate, Rat Brain tissue lysate, Rat Liver tissue lysate All lanes: FUNDC1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa IHC image of CSB-PA816886LA01HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody. Immunofluorescence staining of A549 cell with CSB-PA816886LA01HU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FUNDC1 Polyclonal antibody

Uniprot No.

Q8IVP5

Target Names

FUNDC1

Alternative Names

FUNDC1; FUN14 domain-containing protein 1

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human FUN14 domain-containing protein 1 protein (96-155AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FUNDC1 Antibody (Product code: CSB-PA816886LA01HU) is Non-conjugated. For FUNDC1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA816886LB01HU	FUNDC1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA816886LC01HU	FUNDC1 Antibody, FITC conjugated
Biotin	CSB-PA816886LD01HU	FUNDC1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.02% sodium azide Constituents: 50% Glycerol, PBS, pH 7.3

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:10-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The FUNDC1 antibody was derived from the rabbits immunized with the recombinant human FUNDC1 protein (1-46aa). This rabbit anti-human FUNDC1 polyclonal antibody was demonstrated to recognize human, mouse, and rat FUNDC1 proteins in ELISA and IHC applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity.

The target protein FUNDC1 mainly regulates mitophagy by serving as a receptor that recruits damaged or dysfunctional mitochondria to the autophagosome for degradation. It is also involved in regulating mitochondrial morphology and dynamics, as well as various physiological and pathological processes.

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Target Background

Function

Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.

Gene References into Functions

Our data suggested that FUNDC1 can be used as a prognostic biomarker in patients with cervical cancer, and may be a new therapeutic target to improve the antitumor effects of chemoradiotherapy. PMID: 28719148
phosphorylation of Tyr18 of FUNDC1 serves as a molecular switch for mitophagy. PMID: 27653272
FUNDC1 integrates mitochondrial fission and mitophagy at the interface of the endoplasmic reticulum-mitochondrial contact site by working in concert with DRP1 and calnexin under hypoxic conditions in mammalian cells. PMID: 27145933
FUNDC1 regulates both mitochondrial fission or fusion and mitophagy. PMID: 27050458
The BCL2L1-PGAM5-FUNDC1 axis is critical for receptor-mediated mitophagy. PMID: 25126723
FUNDC1 regulates ULK1 recruitment to damaged mitochondria, where FUNDC1 phosphorylation by ULK1 is crucial for mitophagy. PMID: 24671035
Data show that Knockdown of endogenous FUNDC1 significantly prevented hypoxia-induced mitophagy, which could be reversed by the expression of wild-type FUNDC1. PMID: 22267086

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Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein.

Protein Families

FUN14 family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 28746

OMIM: 300871

KEGG: hsa:139341

STRING: 9606.ENSP00000367284

UniGene: Hs.7549

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The Overview of Autophagy Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Mitophagy

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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