GDI1 Antibody

Datasheet

Code	CSB-PA01229A0Rb
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate All lanes: GDI1 antibody at 6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa IHC image of CSB-PA01229A0Rb diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA01229A0Rb diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA01229A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GDI1 Polyclonal antibody

Uniprot No.

P31150

Target Names

GDI1

Alternative Names

1A antibody; FLJ41411 antibody; GDI-1 antibody; gdi1 antibody; GDIA_HUMAN antibody; GDIL antibody; GDP dissociation inhibitor 1 antibody; Guanosine diphosphate dissociation inhibitor 1 antibody; Mental retardation X-linked 41 antibody; Mental retardation X-linked 48 antibody; MRX41 antibody; MRX48 antibody; Oligophrenin 2 antibody; Oligophrenin-2 antibody; OPHN2 antibody; Protein XAP 4 antibody; Protein XAP-4 antibody; Protein XAP4 antibody; Rab GDI alpha antibody; Rab GDP dissociation inhibitor alpha antibody; RABGD1A antibody; RABGDIA antibody; XAP 4 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Rab GDP dissociation inhibitor alpha protein (35-170AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GDI1 Antibody (Product code: CSB-PA01229A0Rb) is Non-conjugated. For GDI1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA01229B0Rb	GDI1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA01229C0Rb	GDI1 Antibody, FITC conjugated
Biotin	CSB-PA01229D0Rb	GDI1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.

Gene References into Functions

Data confirm isolated adrenocorticotropin deficiency (IAD) as autoimmune disease; RABGDIA (human, human recombinant, or rat) exhibits characteristics of autoantigen in anterior pituitary; patients with IAD exhibit autoantibodies against RABGDIA. PMID: 25346144
Human oligodendrogliomas cells stably expressing mutant GDI1 showed altered cell proliferation. PMID: 25694352
The results of this study identified RabGDI as a component of the switching mechanism that is required for commissural axons to change their response from attraction to repulsion at the intermediate target PMID: 23140504
GDI1 mutations cause an unspecific phenotype characterized by cognitive deficits of variable degree in males, and to a lesser degree in female carriers. PMID: 22002931
The mutations in this gene are common and do not seem to influence the gene expression so as to cause a change in phenotype. PMID: 21736009
A model by which mutant PrP induces overexpression of GDI, activating a cytotoxic feedback loop that leads to protein accumulation in the secretory pathway. PMID: 19996123
RhoGDI has a role in inactivating Rho GTPases with ExoS RhoGAP PMID: 15292224
isoprenylcysteine carboxylmethyltransferase regulates Rac1 activity by controlling the interaction of Rac1 with RhoGDI through TNFA PMID: 15647276
RhoGDI overexpression is associated with paclitaxel resistance in ovarian cancers PMID: 16596196
The results of case-control analysis indicated that, no association between the rs11549300 polymorphisms of GDI1 and children NSMR (P >0.05), but its polymorphisms may be related to intelligence levels of children in Qinba region (P =0.03). PMID: 18487148
GDI-1 is preferentially involved in insulin-stimulated glucose transporter 4 (GLUT4) translocation through facilitating Rab10 recycling. PMID: 19570034

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Involvement in disease

Mental retardation, X-linked 41 (MRX41)

Subcellular Location

Cytoplasm. Golgi apparatus, trans-Golgi network.

Protein Families

Rab GDI family

Tissue Specificity

Brain; predominant in neural and sensory tissues.

Database Links

HGNC: 4226

OMIM: 300104

KEGG: hsa:2664

STRING: 9606.ENSP00000394071

UniGene: Hs.595950

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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