GGCX Antibody

Code CSB-PA009388LA01HU
Size US$166
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  • IHC image of CSB-PA009388LA01HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) GGCX Polyclonal antibody
Uniprot No.
Target Names
GGCX
Alternative Names
GGCX; GC; Vitamin K-dependent gamma-carboxylase; Gamma-glutamyl carboxylase; Peptidyl-glutamate 4-carboxylase; Vitamin K gamma glutamyl carboxylase
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Vitamin K-dependent gamma-carboxylase protein (383-526AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The GGCX Antibody (Product code: CSB-PA009388LA01HU) is Non-conjugated. For GGCX Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA009388LB01HU GGCX Antibody, HRP conjugated ELISA
FITC CSB-PA009388LC01HU GGCX Antibody, FITC conjugated
Biotin CSB-PA009388LD01HU GGCX Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide.
Gene References into Functions
  1. The risk for ischemic atherothrombotic stroke in patients with the gamma-glutamyl carboxylase T/T genotype was higher than in major C-allele carriers. PMID: 29975826
  2. unlike the traditional in vitro GGCX activity assay, allows us to assess the functionality of GGCX using its natural protein substrates in a cellular milieu PMID: 27394683
  3. 2 causative GGCX mutations ( c.1889-6G>A and c.1657delA) from the first reported clinical case of a VKCFD patient. The intronic mutation c.1889-6G>A affects GGCX splicing. Functional characterization of these 2 mutations suggests that truncation of GGCX from its C-terminus affects not only substrate binding but also protein stability. PMID: 28679738
  4. impact of CYP4F2, ABCB1, and GGCX polymorphisms on bleeding episodes associated with acenocoumarol in Russian patients with atrial fibrillation PMID: 27662649
  5. this systematic review suggests that there indeed may be genotype-phenotype correlations for GGCX-related phenotypes, which can guide patient counseling and management. PMID: 28125048
  6. GGCX c.2084+45G polymorphisms has a moderate effect on VKAs dose requirements in Slavic population from Central-Eastern Europe. PMID: 25042728
  7. Detected are ten mutations in the gamma-glutamyl carboxylase gene in patients with hereditary deficiency of vitamin K-dependent coagulation factors. PMID: 25151188
  8. The allele frequency for GGCX 12970 C > G is 1.43 in north Indians and did not have a significant bearing on the maintenance dose of acenocoumarol oral anticoagulant in cardiac valve replacement patients. PMID: 24927344
  9. GGCX mutation found in families with pseudoxanthoma elasticum with retinitis pigmentosa and cutis laxa. PMID: 24739904
  10. These findings indicate that individuals carrying the CYP2C19 rs3814637CC or CYP2C9 rs1057910AA or GGCX rs699664AA genotype needed higher warfarin doses in the Chinese population. PMID: 23941071
  11. In atrial fibrillation population in Xinjiang, patients with CT and TT genotypes in the gamma-glutamyl carboxylase gene rs259251 loci required significantly higher warfarin dose than those with CC genotype. PMID: 24148610
  12. evaluation of urinary Gla excretion in relation with apo E genotype PMID: 23817635
  13. study demonstrated the effects of SNP (974G>A) in the GGCX gene on the correlation between dietary vitamin K intake and gamma-carboxylation of serum osteocalcin PMID: 24231026
  14. there may be no significant association between the low activity and mutation of GGCX in calcium oxalate urolithiasis PMID: 19821094
  15. Quantitative PCR assays for VKORC1, CYP4F2, GGCX and CALU identified two copies in all populations. PMID: 22188360
  16. GGCX polymorphism appeared to have an influence over the reduction of undercarboxylated osteocalcin, especially in older women (age >/=65). PMID: 21344298
  17. no association between haplotypes and venous thrombosis PMID: 21800014
  18. no effects of genetic variants on maintenance warfarin dose in a multi-ethnic Asian population PMID: 21475774
  19. Molecular analysis of the gamma-glutamylcarboxylase gene revealed a heterozygous single nucleotide polymorphism, which decreases carboxylase activity and induces VK-dependent coagulation deficiency. PMID: 21704322
  20. genetic polymorphism affects therapeutic dose of warfarin PMID: 20694283
  21. The activity and expression of GGCX are decreased in renal tissues of patients with calcium oxolate urolithiasis. PMID: 20332604
  22. Subtle polymorphisms, including those in GGCX, NQO1, and VKORC1 genes, influence individual susceptibility to the development of atherosclerotic stroke. PMID: 20193673
  23. Effect of vitamin K-dependent protein precursor propeptide, vitamin K hydroquinone, and glutamate substrate binding on the structure and function of {gamma}-glutamyl carboxylase. PMID: 20716530
  24. Gene polymorphisms of VKORC1 significantly associated with the variation of interindividual warfarin dose requirement variation, and the effects are different in ethnicities. PMID: 19942260
  25. characterization of vitamin K-dependent gamma-glutamyl carboxylase internal propeptide PMID: 12034728
  26. Cys-99 and Cys-450 form the only disulfide bond in carboxylase PMID: 12963724
  27. mutations in residues between 393 and 404 in gamma-glutamyl carboxylase cause impaired glutamate binding PMID: 12968027
  28. A 14-base deletion was found in intron 1 (bases 1056-1069) of the gamma-carboxylase gene. It destroys a reverse palindromic sequence (TTGAGGCAA) of the type often associated with cis-acting elements. This element may regulation the enzyme's expression. PMID: 14567538
  29. GGCX SNP showed a small but significant effect on warfarin dose. PMID: 15883587
  30. Crystallization of human GGCX. PMID: 16979907
  31. report demonstrates the different activities of GGCX between the common genotypes and their association with bone mineral density PMID: 17029979
  32. In addition to polymorphisms in VKORC1 and CYP2C9, we identified GGCX 8016G>A, resulting in the missense mutation R325Q, as a genetic determinant of warfarin maintenance dose in Japanese patients. PMID: 17049586
  33. Mass spectrometric results show that the N-linked glycosylation in carboxylase occurs at positions N459, N550, N605, and N627. PMID: 17144668
  34. identified 37 SNPs in GGCX. The GGCX-12970 SNP had a small, but significant effect, on warfarin maintenance dose PMID: 17764537
  35. There is no significant association between the polymorphisms in GGCX and the warfarin dose requirement. PMID: 17786385
  36. GGCX R325Q genotype did not provide significant differences in acenocoumarol dose requirements in patients PMID: 18234294
  37. A homology model of gamma-glutamyl carboxylase transmembrane domains 2 and 5 suggests that not only do these two domains associate but that transmembrane domain 2 may interact with another transmembrane domain. PMID: 18498174
  38. analysis of GGCX and ABCC6 mutations in a family with pseudoxanthoma elasticum-like phenotypes [case report] PMID: 18800149
  39. Our findings also confirm GGCX as the second gene locus causing Pseudoxanthoma elasticum PMID: 19116367
  40. heterozygous carriers of GGCX rs10187424 and rs7568458 had significantly lower percent undercarboxylated osteocalcin relative to either homozygous group. PMID: 19436136
  41. Polymorphisms in VKORC1 and GGCX are not major genetic determinants of vitamin K-dependent coagulation factor activity in Western Germans. PMID: 19652895
  42. Cys-99 and Cys-450 are free sulfhydryls in the gamma-glutamyl carboxylase active site. The free sulfhydryls were mapped by isolating a native carboxylase-factor IX enzyme substrate complex, modification with NEM and mass spectral mapping. PMID: 11087858
  43. An activated amine initiates the vitamin K-dependent carboxylation reaction, while the Cys-99 and Cys-450 free sulfhydryls play other important roles in the carboxylase reaction PMID: 15365175
  44. Quantitative radiolabeled N-ethylmaleimide modification of a carboxylase with all Cys residues changed to Ala supports the identification of Cys-99 and Cys-450 as the free sulfhydryls in the active site. PMID: 15365175

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Involvement in disease
Combined deficiency of vitamin K-dependent clotting factors 1 (VKCFD1); Pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Vitamin K-dependent gamma-carboxylase family
Database Links

HGNC: 4247

OMIM: 137167

KEGG: hsa:2677

STRING: 9606.ENSP00000233838

UniGene: Hs.77719

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