HPD Antibody

Code CSB-PA010698LA01HU
Size US$166
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Image
  • Western blot
    All lanes: HPD antibody at 4.5µg/ml
    Lane 1: Mouse kidney tissue
    Lane 2: Mouse liver tissue
    Lane 3: HepG2 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 45, 41 kDa
    Observed band size: 45 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010698LA01HU at dilution of 1:20

  • Immunofluorescent analysis of HepG2 cells using CSB-PA010698LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) HPD Polyclonal antibody
Uniprot No.
Target Names
HPD
Alternative Names
4 HPPD antibody; 4 hydroxyphenylpyruvate dioxygenase antibody; 4 hydroxyphenylpyruvic acid oxidase antibody; 4-hydroxyphenylpyruvate dioxygenase antibody; 4-hydroxyphenylpyruvic acid oxidase antibody; 4HPPD antibody; GLOD3 antibody; glyoxalase domain containing 3 antibody; HPD antibody; HPPD_HUMAN antibody; HPPDase antibody; PPD antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human 4-hydroxyphenylpyruvate dioxygenase protein (2-393AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The HPD Antibody (Product code: CSB-PA010698LA01HU) is Non-conjugated. For HPD Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA010698LB01HU HPD Antibody, HRP conjugated ELISA
FITC CSB-PA010698LC01HU HPD Antibody, FITC conjugated
Biotin CSB-PA010698LD01HU HPD Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:10-1:100
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Inoculating a rabbit with recombinant human 4-hydroxyphenylpyruvate dioxygenase protein (2-393aa), extracting antibodies from the rabbit serum, and then purifying the collected antibodies via protein G affinity chromatography. The resulting antibody is the HPD polyclonal antibody. It contains minimal impurities, and its purity exceeds 95%.

This HPD polyclonal is capable of recognizing and binding to HPD protein from human and mouse samples. It has been validated in various experimental settings, including ELISA, WB, IHC, and IF, and can be used for the detection of levels, determination of presence and size, and analysis of the distribution of the HPD protein.

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Target Background

Function
Key enzyme in the degradation of tyrosine.
Gene References into Functions
  1. The mutagenesis and structural simulation studies demonstrate the critical and unique role of each ligand in the function of HPPD, and which correlates with their respective co-ordination position. PMID: 26936969
  2. 4-hydroxyphenylpyruvate dioxygenase gene mutation is associated with Hawkinsinuria. PMID: 26226126
  3. Glutamine375 has a critical role for 4-HPPD in orientating the tail and ensuring the conformation of the terminal alpha-helix of the enzyme to maintain the integrity of the active site for biocatalysis. PMID: 23950902
  4. The IIe335Met allele is equivalent to a null mutation while the Asn241Ser allele results in a partially active enzyme with an uncoupled turnover causing elevated haykinin in urine. PMID: 17560158
Involvement in disease
Tyrosinemia 3 (TYRSN3); Hawkinsinuria (HAWK)
Protein Families
4HPPD family
Database Links

HGNC: 5147

OMIM: 140350

KEGG: hsa:3242

STRING: 9606.ENSP00000289004

UniGene: Hs.2899

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