KARS Antibody

Datasheet

Code	CSB-PA163096
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA163096(KARS Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA163096(KARS Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-6: K562 cells, A549 cells, HT29 cells, 293T cells, Hela cells, Jurkat cells, Primary antibody: CSB-PA163096(KARS Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q15046

Target Names

KARS

Alternative Names

CMTRIB antibody; DFNB89 antibody; EC 6.1.1.6 antibody; KARS 1 antibody; KARS 2 antibody; KARS antibody; KARS1 antibody; KARS2 antibody; KIAA0070 antibody; KRS antibody; Lysine tRNA ligase antibody; Lysine--tRNA ligase antibody; LysRS antibody; Lysyl tRNA synthetase antibody; Lysyl-tRNA synthetase antibody; SYK_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Fusion protein of Human KARS

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:100-1:300

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.; (Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.

Gene References into Functions

The studies showed that mutations in KARS lead to a newly defined subtype of leukoencephalopathy associated with sensorineural hearing impairment. The combined effect of reduced aminoacylation and release of LysRS from the multiple-synthetase complex (MSC) likely underlies the pathogenesis of the KARS mutations identified in this study. PMID: 28887846
Using the TruSight One sequencing panel we identified two novel mutations in KARS. Both mutations, never reported previously, occur in a highly conserved region of the catalytic domain and displayed a dramatic effect on KARS stability PMID: 27891585
Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from colorectal cancer cells. PMID: 28611052
Results indicate that KRS can promote cell-cell and cellextracellular matrix adhesion for migration. PMID: 26891990
finding show that enzymatically active Shiga toxins trigger the dissociation of lysyl-tRNA synthetase (KRS) from the multi-aminoacyl-tRNA synthetase complex in human macrophage-like differentiated THP-1 cells and its subsequent secretion. PMID: 26643967
KRS at the plasma membrane plays new roles in metastatic migration as a signaling inducer, and causes intracellular signaling for cancer dissemination PMID: 26091349
tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939
structural characteristics of the KRS-LR interaction on the cell surface PMID: 24983501
Lysyl-tRNA synthetase plays essential role in HIV replication, transcriptional regulation, cytokine-like signaling. [review] PMID: 23972532
The role of preKARS2 in the tRNA mitochondrial import. PMID: 23799079
The KARS variant is identified in two families affected by DFNB89-associated autosomal-recessive nonsyndromic hearing impairment. PMID: 23768514
C-terminal domain of HIV-1 capsid protein as surrogate for human lysyl tRNA synthetase PMID: 23208549
A single conformational change triggered by phosphorylation leads to multiple effects driving an exclusive switch of LysRS function from translation to transcription. PMID: 23159739
Dual role for motif 1 residues of human lysyl-tRNA synthetase in dimerization and packaging into HIV-1. PMID: 23095741
The work thus unveiled a unique function of KRS in the control of cell migration and its pathological implication in metastasis. PMID: 22751010
Data are consistent with hypothesis that maturation of cytoplasmic KARS precursor is needed to reveal the potent tRNA binding properties of mitochondrial KARS. PMID: 22235746
LysRS associates with the Pol domain of GagPol. PMID: 21763493
The results suggest that this unique geometry, which reconfigures the LysRS tetramer from alpha(2):alpha(2) to alpha(2)beta(1):beta(1)alpha(2), is designed to control both retention and mobilization of LysRS from the multi-tRNA synthetase complex PMID: 21536907
The interaction between helix 7 of LysRS and helix 4 of the capsid C-terminal domain of HIV-1 Gag (HIV-CA-CTD) was studied using circular dichromism spectroscopy and molecular dynamics simulation. PMID: 21093454
These results underscore the contribution of KARS to the emission of (one of) the principal signal(s) of immunogenic cell death, CRT exposure. PMID: 20699648
Loss-of-function lysyl-tRNA synthetase mutations associated with peripheral neuropathy and Charcot-Marie-Tooth disease. PMID: 20920668
HIV-1 Gag interacts with human lysyl-tRNA synthetase during viral assembly PMID: 12756246
packaged into human immunodeficiency virus type 1 (HIV-1) via its interaction with Gag; this enzyme facilitates the selective packaging of tRNA(3)(Lys), the primer for initiating reverse transcription, into HIV-1 PMID: 15220430
analysis of the interaction between HIV-1 Gag and human lysyl-tRNA synthetase PMID: 16702215
HIV-1 Vpr fulfills an essential role in the process of packaging of mitochondrial Lysyl-tRNA synthase. PMID: 17560997
This work reports further characterization of the interaction between HIV-1 capsid domain of Gag and human LysRS using truncation constructs and point mutations in the putative interaction helices. PMID: 17724017
present a 2.3-A crystal structure of a tetrameric form of human LysRS PMID: 18272479
mitoKARS is the first described member of a group of mitochondrial proteins whose interaction with mutant SOD1 contributes to mitochondrial dysfunction in ALS PMID: 18715867

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Involvement in disease

Charcot-Marie-Tooth disease, recessive, intermediate type, B (CMTRIB); Deafness, autosomal recessive, 89 (DFNB89)

Subcellular Location

[Isoform Cytoplasmic]: Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted.; [Isoform Mitochondrial]: Mitochondrion.

Protein Families

Class-II aminoacyl-tRNA synthetase family

Database Links

HGNC: 6215

OMIM: 601421

KEGG: hsa:3735

STRING: 9606.ENSP00000325448

UniGene: Hs.3100

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