PLCZ1 Antibody

Code CSB-PA768240LA01HU
Size US$166
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  • Western blot
    All lanes: PLCZ1 antibody at 3µg/ml
    Lane 1: Mouse liver tissue
    Lane 2: Mouse kidney tissue
    Lane 3: Human placenta tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 71, 48, 58 kDa
    Observed band size: 71 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PLCZ1 Polyclonal antibody
Uniprot No.
Target Names
PLCZ1
Alternative Names
1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1 antibody; 1-phosphatidylinositol-4 antibody; 5-bisphosphate phosphodiesterase zeta-1 antibody; MGC149685 antibody; NYD SP27 antibody; Phosphoinositide phospholipase C zeta 1 antibody; Phosphoinositide phospholipase C-zeta-1 antibody; Phospholipase C zeta 1 antibody; Phospholipase C-zeta-1 antibody; PI phospholipase C zeta 1 antibody; PLC zeta 1 antibody; PLC-zeta-1 antibody; PLCZ1 antibody; PLCZ1_HUMAN antibody; PLCzeta antibody; Testis development protein NYD SP27 antibody; Testis development related NYD SP27 antibody; Testis-development protein NYD-SP27 antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 protein (1-415AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The PLCZ1 Antibody (Product code: CSB-PA768240LA01HU) is Non-conjugated. For PLCZ1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA768240LB01HU PLCZ1 Antibody, HRP conjugated ELISA
FITC CSB-PA768240LC01HU PLCZ1 Antibody, FITC conjugated
Biotin CSB-PA768240LD01HU PLCZ1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description
PLCZ1 polyclonal antibody CSB-PA768240LA01HU was produced in rabbit immunized by using the Recombinant Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 protein (1-415AA) as the immunogen. The target protein PLCZ1, a member of the phosphoinositide-specific phospholipase C family, localizes to the acrosome in spermatozoa and elicits Ca2+ oscillations and egg activation during fertilization that leads to early embryonic development. Diseases associated with PLCZ1 include Spermatogenic Failure 17 and Spermatogenic Failure 9.
This Rabbit anti-Homo sapiens (Human) PLCZ1 Polyclonal antibody was tested in the ELISA and WB applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the PLCZ1 proteins of human or mouse-origin and may be used to detect the endogenous levels of PLCZ1 protein.

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Target Background

Function
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.
Gene References into Functions
  1. PLCzeta and PAWP impairments may be one of the possible etiologies of decreased fertility in Oligoasthenoteratozoospermia PMID: 28954204
  2. Low PLCZ1 expression is associated with globozoospermia with DPY19L2 deletion. PMID: 29339016
  3. we evaluate sperm motility and the proportion of sperm expressing PLC zeta in 71 males (22-54 years; 44 fertile controls and 27 infertile patients), along with total levels and localisation patterns of PLC zeta within the sperm head. PMID: 27270687
  4. findings suggest that human PLCZ1 RNA is a better therapeutic agent to rescue human oocytes from failed activation, leading to normal and efficient development. PMID: 28320563
  5. The value of PAWP and PLCzeta as indicators of sperm quality was studied. PMID: 28076980
  6. The role of the PLCZ1 protein in male infertility, and its expression regulation and therapeutic potential have been discussed. (Review) PMID: 26700242
  7. latest developments that have begun to unravel the vital role of PLCzeta at mammalian fertilisation and decipher its unique mechanism of action within the fertilising egg. PMID: 29061915
  8. The findings highlight the importance of PLCzeta at fertilization and the vital role of the C2 domain in PLCzeta function, possibly due to its novel binding characteristics. PMID: 28270562
  9. The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCzeta PMID: 27612872
  10. In male infertility, the absence of PLCZ1 alone is sufficient to prevent oocyte activation irrespective of the presence of PAWP. PMID: 26721930
  11. Human PLCzeta elicited the characteristic Ca(2+) oscillations present at mammalian fertilization, which produced oocyte activation and embryo development. PMID: 26116451
  12. Study further supports the fundamental role of PLCzeta in the oocyte activation process. PMID: 26054556
  13. Phospholipase C-zeta deficiency is associated with repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization. PMID: 26174123
  14. I discuss the recent advances in our knowledge of the intriguing biochemical and physiological properties of sperm PLCz and postulate potential roles for PLCz in terms of clinical diagnosis and therapy for certain forms of male infertility PMID: 26009178
  15. Data suggest that PAWP (WW domain-binding protein 2-like) from sperm (rather than PLCZ [phospholipase C zeta] from sperm) acts as the 'sperm factor' within the egg at fertilization and is involved in 'egg activation' and embryogenesis. [REVIEW] PMID: 25722320
  16. in patients with normal SA parameters but with repeated low fertilization or outright failed fertilization results after ICSI, abnormal PLCZ1 function should be considered as the underlying mechanism responsible for the failure of fertilization PMID: 24756570
  17. Human PLCzeta exhibits superior fertilization potency over mouse PLCzeta in triggering the Ca(2+) oscillations required for mammalian oocyte activation. PMID: 24478462
  18. Male infertility is associated with the aberrant expression, localisation, structure and function of PLCzeta in human sperm. [review] PMID: 23916605
  19. PLCzeta may be a significant factor in human fertility with potential therapeutic capacity. [Review] PMID: 24157362
  20. investigation of expression level and localization pattern of PLCZ1 in sperm from three infertile patients with globozoospermia (i.e., patients who exhibited abnormal sperm with round heads) [CASE REPORT] PMID: 22940771
  21. Loss-of-activity mutations in PLCzeta may contribute not only toward male infertility but also male subfertility in cases where PLCzeta is mutated on a single allele. PMID: 22633260
  22. PLCzeta is compartmentalized as part of the acrosome early in human and mouse spermiogenesis and is secreted during sperm maturation in the epididymis. PMID: 22428063
  23. Phospholipase C-zeta-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection. PMID: 22217962
  24. a maternally inherited autosomal point mutation in human phospholipase C zeta (PLCzeta) leads to male infertility PMID: 22095789
  25. PLCzeta may have a role in sperm inducing oocyte activation after ICSI PMID: 21896550
  26. Male infertility-linked point mutation disrupts the Ca2+ oscillation-inducing and PIP(2) hydrolysis activity of sperm PLC gamma. PMID: 21204786
  27. confirm the previously proposed inhibitory role of NYD-SP27 in the PLC pathway and demonstrate that the suppression of its expression could result in an enhancement of ATP-stimulated Ca(2+) dependent pancreatic anion secretion. PMID: 17196844
  28. narrow spectrum of PLCZ1 activity indicates that it is modulated by tissue-restricted accessory factors PMID: 17933795
  29. Human PLCzeta can readily activate mouse oocytes, however, effective development to blastocyst stages is only achieved within a specific window of hPLCzeta-luc protein expression levels. PMID: 18003622
  30. All PLCZ1 proteins including fish could induce Ca(2+) oscillations in mouse eggs, but the activity was variable in the order of human >> mouse > medaka >> rat, estimated from minimal RNA concentration to induce Ca(2+) spikes. PMID: 18322275
  31. Human sperm devoid of PLCZ1 fail to induce Ca(2+) release and are unable to initiate the first step of embryo development. PMID: 18924610

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Involvement in disease
Spermatogenic failure 17 (SPGF17)
Subcellular Location
Nucleus. Cytoplasm, perinuclear region.
Tissue Specificity
Expressed specifically in testis and sperm. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.
Database Links

HGNC: 19218

OMIM: 608075

KEGG: hsa:89869

STRING: 9606.ENSP00000266505

UniGene: Hs.743832

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