PPM1D Antibody

Code CSB-PA018491ESR2HU
Size US$166
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  • Western blot
    All lanes: PPM1Dantibody at 2.66μg/ml
    Lane 1: Rat gonadal tissue
    Lane 2: U87 whole cell lysate
    Lane 3: 293T whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 67, 48 kDa
    Observed band size: 67 kDa

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA018491ESR2HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA018491ESR2HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PPM1D Polyclonal antibody
Uniprot No.
Target Names
PPM1D
Alternative Names
EC 3.1.3.16 antibody; p53 induced protein phosphatase 1 antibody; p53-induced protein phosphatase 1 antibody; PP2C delta antibody; PP2C-delta antibody; PP2CD antibody; Ppm1d antibody; PPM1D_HUMAN antibody; Protein phosphatase 1D antibody; protein phosphatase 1D magnesium-dependent delta isoform antibody; Protein phosphatase 2C delta isoform antibody; Protein phosphatase 2C isoform delta antibody; Protein phosphatase magnesium dependent 1 delta antibody; Protein phosphatase magnesium-dependent 1 delta antibody; Protein phosphatase Mg2+/Mn2+ dependent 1D antibody; Protein phosphatase Wip1 antibody; Wild type p53 induced phosphatase 1 antibody; WIP 1 antibody; WIP1 antibody
Raised in
Rabbit
Species Reactivity
Human, Rat
Immunogen
Recombinant Human Protein phosphatase 1D protein (1-250AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.
Gene References into Functions
  1. The observations suggest a novel role of ATR kinase in mediating its own signal attenuation via PPM1D recruitment to chromatin as an essential mechanism for restarting the stalled forks, cell cycle re-entry and cellular recovery from replication stress. PMID: 29485113
  2. NF-kappaB served as a positive transcriptional regulator of WIP1 to activate its expression and affect its function in colorectal cancer cells. PMID: 29367109
  3. ese results enhance our understanding of the molecular mechanisms that govern nucleoli formation by demonstrating that PPM1D regulates nucleolar formation by regulating NPM phosphorylation status through a novel signalling pathway, PPM1D-CDC25C-CDK1-PLK1 PMID: 27619510
  4. These data support the notion that Wip1 contributes to the formation of the ERMs in PDR membranes via NF-kappaB signaling. PMID: 28402943
  5. In an in vitro model of the blood-brain barrier, LPS raised Wip1 expression. Wip1 knockdown increased permeability and decreased transepithelial electrical resistance, protein expression of ZO-1, and occluding. Wip1 silencing augmented TNF-alpha, IL-1beta, IL-12, and IL-6. Sonic hedgehog signaling was activated by Wip1 overexpression and inhibited by silencing. Wip1 may protect the BBB against LPS damage via SHH signal... PMID: 29128669
  6. Studies suugest Wip1 role in tumorigenesis through regulation of p53 and p38MAPK pathways. PMID: 26883196
  7. The inhibition of Wip1 might fortify p53-mediated tumor suppression by Mdm2 antagonists. PMID: 27183917
  8. Wip1 suppressed ovarian cancer cell invasion, migration, epithelial to mesenchymal transition (EMT), and ovarian cancer metastasis in xenograft animal models. PMID: 27121065
  9. model reproduces the observed cellular phenotypes in experiments: oscillatory (for low DNA damage) regulated by negative feedback loops involving mainly p53 and Mdm2 and apoptotic or senescent (for high DNA damage) regulated by the positive p53/Wip1/miR-16 feedback loop PMID: 28968438
  10. This suggests an important cross-talk between SHH and WIP1 pathways that accelerates tumorigenesis and supports WIP1 inhibition as a potential treatment strategy for MB. PMID: 27086929
  11. Wip1 activity and its relevance to cancer as an oncoprotein is reviewed PMID: 28385459
  12. The authors show that a global spread of ATM activity on chromatin and phosphorylation of ATM targets including KAP1 control Plk1 re-activation. These phosphorylations are rapidly counteracted by the chromatin-bound phosphatase Wip1, allowing cell cycle restart despite persistent ATM activity present at DNA lesions. PMID: 28607002
  13. Truncating and missense PPM1D mutations are associated with prostate cancer. PMID: 27401275
  14. this study shows that expression of Wip1 is decreased in patients with active inflammatory bowel disease PMID: 27687530
  15. Wip1 is frequently overexpressed in nonsmall cell lung cancer, which may serve an essential role in the p38MAPK/p53/p16 signaling pathway. PMID: 27959454
  16. Study identified 14 individuals with mild to severe Intellectual Disability (ID) and/or developmental delay and de novo truncating PPM1D mutations; all mutations were located in the last or penultimate exon, suggesting escape from nonsense-mediated mRNA decay. PMID: 28343630
  17. PPM1D gene silencing combined with TMZ eradicates glioma cells through cell apoptosis and cell cycle arrest PMID: 27633132
  18. High PPM1D expression is associated with breast cancer. PMID: 27485113
  19. PPM1D in the chromosomal location 17q22-24 might be the most relevant candidate gene with respect to a potential functional implication in meningioma progression. PMID: 26942600
  20. potential prognostic marker and therapeutic target for hepatocellular carcinoma PMID: 26809466
  21. PPM1D suppresses pancreatic cancer cell apoptosis via inhibition of the p38 MAPK/p53 pathway through both dephosphorylation of p38 MAPK and downregulation of ASPP2. PMID: 26714478
  22. squalene inhibits the ATM-dependent signaling pathway following DNA damage through intracellular induction of Wip1 expression. PMID: 26824362
  23. Chemotherapy exposure and age influence the accumulation of PPM1D mutations in the peripheral blood mononuclear cells of ovarian cancer patients. PMID: 26847329
  24. Knockdown of Wip1 enhances sensitivity to radiation in HeLa cells by inhibiting cell proliferation and inducing apoptosis through activation of p38 MAPK. PMID: 26351212
  25. Results showed evidence that Wip1 underwent Cdh1-dependent proteolysis during mitosis and sustained Wip1 activity during mitosis, resulting in mitotic delay at the metaphase to anaphase transition. PMID: 25649870
  26. Study reveals a mechanism of stem cell aging, in which distinct effects of p53 and mTORC1 pathways on HSC aging are governed by Wip1. PMID: 25879755
  27. WIP1 silencing reduced expression of MMP-9, VEGF-C, cyclin D1, and c-Myc, as well as migration and invasion of salivary adenoid cystic carcinoma cells. WIP1 expression was positively associated with ACC metastasis and prognosis. PMID: 25797250
  28. Wip1 may be involved in the biological processes of nasopharyngeal cancer cell proliferation, apoptosis, and migration and invasion. PMID: 25060857
  29. p21 might contribute to positive regulation of WIP1, resulting in dephosphorylation of the histone H2A variant gammaH2AX. PMID: 26006237
  30. PPM1D plays an important role in promoting CDDP resistance. PMID: 25154814
  31. data suggests that WIP1 inhibition represents a novel therapeutic approach to neuroblastoma that could be integrated with current chemotherapeutic approaches PMID: 25658463
  32. ATM kinase and Wip1 phosphatase were identified as opposing regulators of DAXX-S564 phosphorylation and the role of DAXX phosphorylation and DAXX itself are independent of p53-mediated gene expression. PMID: 25659035
  33. Wip1 protein was increased in kidney carcinoma. PMID: 24970693
  34. Results show that downregulation of HIPK2 increases resistance of bladder cancer cell to cisplatin by regulating Wip1. PMID: 24846322
  35. PPM1D plays an important role in the progression of NSCLC. PMID: 25412952
  36. High PPM1D expression is associated with lung cancer. PMID: 25123458
  37. The truncating mutations of PPM1D are present in blood DNA of NSCLC patients. PMID: 25742468
  38. Results demonstrate increased WIP1 expression in metastatic medulloblastomas, and inferior survival in patients with WIP1 high-expressing medulloblastoma. PMID: 24632620
  39. WIP1 knockdown inhibited the invasiveness of CNE-2 and 5-8F cells and was associated with the down-regulation of the expression of matrix metallopeptidase 9 PMID: 24801909
  40. Gain-of-function PPM1D mutations are associated with brainstem gliomas. PMID: 24880341
  41. High PPM1D expression is associated with prostate cancer. PMID: 24922655
  42. PP4 and Wip1 are differentially required to counteract the p53-dependent cell cycle arrest in G1 and G2, by antagonizing early or late p53-mediated responses, respectively PMID: 24711418
  43. Wip1 may be involved in the biological processes of non small cell lung cancer cell proliferation, cell apoptosis, and cell cycle. PMID: 24272082
  44. PPM1D plays a crucial role in colorectal carcinoma cell proliferation and colony formation. PMID: 23745790
  45. PPM1D mutations in the mosaic state predispose women to breast and ovarian cancer in the absence of a family history of cancer PMID: 24262437
  46. Expression of Wip1 in neutrophils was downregulated quickly after neutrophil activation through JNK-microRNA-16 pathway. It was negatively correlated with inflammatory cytokine productions of neutrophils in sepsis patients. PMID: 24395919
  47. Genetic variants and mutations of PPM1D control the response to DNA damage. PMID: 23907125
  48. BAX dephosphorylation of Wip1 phosphatase is an important regulator of resistance to anticancer therapy. PMID: 23907458
  49. High WIP1 expression is associated with colorectal cancer. PMID: 23679302
  50. Wip1 plays an important role in the regulation of global heterochromatin silencing and thus is critical in maintaining genome integrity PMID: 24135283

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Involvement in disease
Intellectual developmental disorder with gastrointestinal difficulties and high pain threshold (IDDGIP); Breast cancer (BC); Ovarian cancer (OC)
Subcellular Location
Nucleus. Cytoplasm, cytosol.
Protein Families
PP2C family
Tissue Specificity
Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.
Database Links

HGNC: 9277

OMIM: 114480

KEGG: hsa:8493

STRING: 9606.ENSP00000306682

UniGene: Hs.286073

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