RNF183 Antibody

Code CSB-PA019862LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA019862LA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA019862LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RNF183 Polyclonal antibody
Uniprot No.
Target Names
RNF183
Alternative Names
RNF183; E3 ubiquitin-protein ligase RNF183
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human E3 ubiquitin-protein ligase RNF183 protein (1-161AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RNF183 Antibody (Product code: CSB-PA019862LA01HU) is Non-conjugated. For RNF183 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA019862LB01HU RNF183 Antibody, HRP conjugated ELISA
FITC CSB-PA019862LC01HU RNF183 Antibody, FITC conjugated
Biotin CSB-PA019862LD01HU RNF183 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Acts as a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Triggers apoptosis in response to prolonged ER stress by mediating the polyubiquitination and subsequent proteasomal degradation of BCL2L1. May collaborate with FATE1 to restrain BIK protein levels thus regulating apoptotic signaling.
Gene References into Functions
  1. RNF183 plays an important role in executing programmed cell death upon prolonged endoplasmic reticulum stress, likely by inducing apoptosis through Bcl-xL.RNF183 ubiquitinates Bcl-xL. PMID: 29507230
  2. RNF183 promotes proliferation and metastasis of colorectal cancer cells via activation of NF-kappaB-IL-8 axis PMID: 28796265
  3. The RNF183-IL-8 axis is responsible for the resistance of colorectal cancer (CRC)cells to the MEK1/2 inhibitor trametinib and may serve as a candidate target for combined therapy for CRC. PMID: 28756770
  4. Sec16A also stabilized the interacting ubiquitin ligase RNF152, which localizes to the lysosome and has structural similarity with RNF183 PMID: 29300766
  5. RNF183, which is negatively regulated by miR-7, is a novel regulator promoting intestinal inflammation by increasing the ubiquitination and degradation of IkappaBalpha, thereby inducing NF-kappaB activation. The interaction between RNF183-mediated ubiquitination and miRNA may be an important novel epigenetic mechanism in the pathogenesis of inflammatory bowel disease. PMID: 26818663

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Endoplasmic reticulum. Golgi apparatus, cis-Golgi network membrane. Lysosome membrane.
Tissue Specificity
Kidney and testis.
Database Links

HGNC: 28721

KEGG: hsa:138065

UniGene: Hs.729641

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