SF1 Antibody

Datasheet

Code	CSB-PA004139
Size	US$100
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Image	Western Blot analysis of 293 cells using Splicing factor 1 Polyclonal Antibody
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Product Details
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Target Background

Product Details

Uniprot No.

Q15637

Target Names

SF1

Alternative Names

SF1 antibody; ZFM1 antibody; ZNF162 antibody; Splicing factor 1 antibody; Mammalian branch point-binding protein antibody; BBP antibody; mBBP antibody; Transcription factor ZFM1 antibody; Zinc finger gene in MEN1 locus antibody; Zinc finger protein 162 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthesized peptide derived from Human Splicing factor 1 around the non-phosphorylation site of S82.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, IF, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:20000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

Gene References into Functions

SF1 Phosphorylation Enhances Specific Binding to U2AF(65) and Reduces Binding to 3'-Splice-Site RNA PMID: 28002734
A novel function of SF1 in the initial recruitment of the U2 snRNP through direct interactions with two U2 snRNP-associated proteins. PMID: 26420826
Data suggest that post-translational processing of SF1 (phosphorylation of Ser20) down-regulates nuclear import of SF1 via alterations in kinetic interaction of SF1 nuclear localization signal (NLS) with NLS receptor isoforms. PMID: 25043850
We demonstrated that PRPF40B interacts directly with SF1 and associates with U2AF(65 PMID: 25605964
Gomafu indirectly modulates the function of the splicing factors SF1 and Celf3 by sequestering these proteins into separate nuclear bodies. PMID: 25145264
The conserved SPSP motif phosphorylation and the SF1/U2AF interface are essential in vivo. PMID: 23273425
Findings suggest that Zinc finger motif-1 (ZFM1) is an important factor for the stabilization of a contractile SMC phenotype under basal or mildly activating conditions. PMID: 22906951
central ;mystery' domain of SF1 crystals belonged to space group C2 and have most probable solvent contents of 64, 52 or 39% with three, four or five molecules per asymmetric unit, respectively PMID: 21505248
SF1 silencing affected alternative splicing of endogenous transcripts, establishing a previously unexpected role for SF1 and branch site-like sequences in splice site selection. PMID: 21062807
the conformational changes that are induced by assembly of the SF1/U2AF(65)/RNA complex serve to position the pre-mRNA splice site optimally for subsequent stages of splicing. PMID: 21146534
SF3a60, 66, and 120, but not SF1, are essential for pre-mRNA splicing PMID: 15647371
SF1 was essential for the induction of alternative mRNA splicing by the beta-catenin/TCF4 complex. PMID: 17383426
Puf60-UHM binds to ULM sequences in the splicing factors SF1, U2AF65, and SF3b155. PMID: 18974054
The KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for intron branched point sequence (BPS) binding. PMID: 11691992

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Subcellular Location

Nucleus.

Protein Families

BBP/SF1 family

Tissue Specificity

Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.

Database Links

HGNC: 12950

OMIM: 601516

KEGG: hsa:7536

STRING: 9606.ENSP00000366604

UniGene: Hs.502829

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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