SLC30A10 Antibody

Datasheet

Code	CSB-PA747695LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: SY5Y whole cell lysate(20µg), COLO205 whole cell lysate(20µg), Mouse Brain tissue lysate(20µg) All lanes: SLC30A10 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 53,27,25 kDa Observed band size: 53 kDa Exposure time: 120s IHC image of CSB-PA747695LA01HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody IHC image of CSB-PA747695LA01HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SLC30A10 Polyclonal antibody

Uniprot No.

Q6XR72

Target Names

SLC30A10

Alternative Names

SLC30A10; ZNT10; ZNT8; Zinc transporter 10; ZnT-10; Manganese transporter SLC30A10; Solute carrier family 30 member 10

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Zinc transporter 10 protein (58-278AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SLC30A10 Antibody (Product code: CSB-PA747695LA01HU) is Non-conjugated. For SLC30A10 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA747695LB01HU	SLC30A10 Antibody, HRP conjugated	ELISA
FITC	CSB-PA747695LC01HU	SLC30A10 Antibody, FITC conjugated
Biotin	CSB-PA747695LD01HU	SLC30A10 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SLC30A10 antibody is a premium polyclonal antibody used to detect the SLC30A10 protein of human and mouse origin. The recombinant human zinc transporter 10 protein (58-278AA) is used as the immunogen to immunize the rabbit to obtain the SLC30A10 antibody. This unconjugated SLC30A10 antibody exists as an IgG isoform. It is purified using protein G and reaches up to 95% in purity. It has been validated for use in four applications, including ELISA, WB, IHC, and IF. Its target protein SLC30A10 protects cells from manganese ion toxicosis by clearing out manganese when sensing the increased manganese levels. In the liver cells, the SLC30A10 protein removes the manganese by transporting it out of the cells into bile.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays a pivotal role in manganese transport. Manganese is an essential cation for the function of several enzymes, including some crucially important for the metabolism of neurotransmitters and other neuronal metabolic pathways. However, elevated levels of manganese are cytotoxic and induce oxidative stress, mitochondrial dysfunction and apoptosis. Acts as manganese efflux transporter and confers protection against manganese-induced cell death. Also acts as zinc transporter involved in zinc homeostasis. Seems to mediate zinc transport into early endosomes and recycling endosomes to prevent zinc toxicity; the function may be regulated by heterodimerization with other zinc transporters of the SLC30A subfamily. The SLC30A3:SLC30A10 heterodimer is involved in zinc transport-dependent regulation of the EGFR/ERK transduction pathway in endosomes. May be involved in regulation of zinc-dependent senescence of vascular smooth muscle cells.

Gene References into Functions

Structural homology analysis provide evidence that L349P mutation severe structural changes of ZnT-10 in its CTD domain resulting in abnormal reduced function. PMID: 27550551
SLC30A10 has a protective role in 1-methyl-4-phenylpyridinium-induced toxicity via PERK-ATF4 pathway. PMID: 28688763
xpressing either wild-type or mutant forms of SLC30A10 was sufficient to inhibit the effect of ATP2C1 in response to Mn challenge in both zebrafish embryos and HeLa cells. These findings suggest that either activating ATP2C1 or restoring the Mn-induced trafficking of ATP2C1 can reduce Mn accumulation, providing a possible target for treating HMDPC. PMID: 28692648
results indicate that residues in the transmembrane and C-terminal domains together confer optimal manganese transport capability to SLC30A10 and suggest that the mechanism of ion coordination in the transmembrane domain of SLC30A10 may be substantially different from that in YiiP/other SLC30 proteins. PMID: 27307044
allele was also associated with increased sway velocity (15%, P = .033; adjusted for age and sex) and reduced SLC30A10 expression PMID: 26628504
We describe early disease manifestations (including videos) in 5 previously unreported Indian children, carrying novel homozygous SLC30A10 mutation PMID: 25778823
SLC30A10 is a cell surface-localized manganese efflux transporter that reduces manganese levels and protects against manganese toxicity. PMID: 25319704
These results suggest that both the up-regulation of ZIP14 and the down-regulation of ZnT10 by IL-6 might have enhanced the accumulation of manganese in SH-SY5Y cells. PMID: 24576911
a case of inherited manganism caused by SLC30A10 mutation is presented PMID: 23369405
ZnT10 is significantly decreased in the frontal cortex in Alzheimer's disease. PMID: 23741496
This review will address Mn transport proteins, the newly discovered SLC30A10 mutations and their implications to Parkinsonism and Mn regulation. PMID: 23357421
The study demonstrates down-regulation by Zn of ZnT10 mRNA levels in cultured intestinal and neuroblastoma cell lines and demonstrate reduced transcription from the ZnT10 promoter at an elevated extracellular Zn concentration. PMID: 22706290
SLC30A10 mutations cause a treatable recessive disease with pleomorphic phenotype, and provide compelling evidence that SLC30A10 plays a pivotal role in manganese transport. PMID: 22341971
This work has confirmed that SLC30A10 functions as a Mn transporter in humans that, when defective, causes Mn accumulation in liver and brain. PMID: 22341972
identified the full-length sequences of SLC30A10, extending the SLC30 family to ten members; used an expressed sequence tag (EST) data mining strategy to determine the pattern of ZnT genes expression in tissues PMID: 15154973

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Involvement in disease

Hypermanganesemia with dystonia 1 (HMNDYT1)

Subcellular Location

Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network. Recycling endosome. Early endosome.

Protein Families

Cation diffusion facilitator (CDF) transporter (TC 2.A.4) family, SLC30A subfamily

Tissue Specificity

Specifically expressed in fetal liver and fetal brain. Expressed in adult tissues with relative levels small intestine > liver > testes > brain > ovary > colon > cervix > prostate > placenta.

Database Links

HGNC: 25355

OMIM: 611146

KEGG: hsa:55532

STRING: 9606.ENSP00000355893

UniGene: Hs.284450

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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