TBCB Antibody

Code CSB-PA03169A0Rb
Size US$166
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Image
  • Western blot
    All lanes: TBCB antibody at 4µg/ml
    Lane 1: Hela whole cell lysate
    Lane 2: A549 whole cell lysate
    Lane 3: Jurkat whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 28, 22 kDa
    Observed band size: 28 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03169A0Rb at dilution of 1:100

  • Immunoprecipitating TBCB in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA03169A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
    Lane 2: CSB-PA03169A0Rb (4µg) + Hela whole cell lysate (500µg)
    Lane 3: Hela whole cell lysate (20µg)

  • Immunofluorescent analysis of Hela cells using CSB-PA03169A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TBCB Polyclonal antibody
Uniprot No.
Target Names
TBCB
Alternative Names
CG22 antibody; CKAPI antibody; Cytoskeleton associated protein 1 antibody; Cytoskeleton associated protein CKAPI antibody; Cytoskeleton-associated protein 1 antibody; Cytoskeleton-associated protein CKAPI antibody; Tbcb antibody; TBCB_HUMAN antibody; Tubulin folding cofactor B antibody; Tubulin specific chaperone B antibody; Tubulin-folding cofactor B antibody; Tubulin-specific chaperone B antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Tubulin-folding cofactor B protein (1-244AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The TBCB Antibody (Product code: CSB-PA03169A0Rb) is Non-conjugated. For TBCB Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA03169B0Rb TBCB Antibody, HRP conjugated ELISA
FITC CSB-PA03169C0Rb TBCB Antibody, FITC conjugated
Biotin CSB-PA03169D0Rb TBCB Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Customer Reviews and Q&A

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Applications : WB

Sample type: Human HEK293 cells

Review: The downregulation of TBCB and CASP3 as observed by mass spectrometry could be confirmed in mimic-1825 transfected HEK293 cells by western blotting, while levels of RPAB3 did not change.

By Anonymous

Target Background

Function
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
Gene References into Functions
  1. Data indicate a microRNA-1825/TBCB/TUBA4A pathway as a putative pathogenic cascade in both familial ALS (fALS) and both sporadic (sALS). PMID: 30030593
  2. the role of the human TBCE and TBCB chaperones in alpha-tubulin-beta-tubulin dissociation, was investigated. PMID: 25908846
  3. results support a role for MAP1 proteins in promoting efficient retrograde trafficking of HIV-1 by stimulating the formation of stable microtubules and mediating the association of HIV-1 cores with microtubules. PMID: 25505242
  4. Overexpression of TBCB leads to the decreased localization of p150 to the microtubule network that might result in a functional modulation of this protein complex. PMID: 22777741
  5. TBCB localizes at spindle and midzone microtubules during mitosis. PMID: 22940919
  6. These findings identified TCoB as the third cytoskeleton protein to be nitrated and suggest a previously undescribed mechanism, whereby growth factor signaling may coordinately integrate nitric oxide signaling in the regulation of microtubule dynamics. PMID: 18048340
  7. TBCB functions as a microtubule density regulator in microglia during activation, and provide an insight into understanding of mechanisms controlling microtubule reorganization during microglial transition between phenotypes. PMID: 19038251
  8. Study demonstrates that, unlike its counterpart TBCE, TBCB only moderately destabilizes microtubules; neither TBCB abundance nor microtubule organization or densities are altered in giant axonal neuropathy mutant fibroblasts. PMID: 19168853

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton.
Protein Families
TBCB family
Tissue Specificity
Found in most tissues.
Database Links

HGNC: 1989

OMIM: 601303

KEGG: hsa:1155

STRING: 9606.ENSP00000221855

UniGene: Hs.31053

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