USE1 Antibody

Datasheet

Code	CSB-PA889157LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Jurkat whole cell lysate, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue, Mouse skeletal muscle tissue All lanes: USE1 antibody at 2.8μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30, 17, 16 kDa Observed band size: 36 kDa Immunofluorescent analysis of U251 cells using CSB-PA889157LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunoprecipitating USE1 in PC-3 whole cell lysate Lane 1: Rabbit control IgG (1μg) instead of CSB-PA889157LA01HU in PC-3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA889157LA01HU (6μg) + PC-3 whole cell lysate (500μg) Lane 3: PC-3 whole cell lysate (10μg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) USE1 Polyclonal antibody

Uniprot No.

Q9NZ43

Target Names

USE1

Alternative Names

2010315L10Rik antibody; 5730403H22Rik antibody; AV002165 antibody; D12 antibody; Ed2 antibody; Embryonic development factor 2 antibody; MDS032 antibody; P31 antibody; Protein D12 antibody; Protein p31 antibody; Putative MAPK activating protein PM26 antibody; Putative MAPK-activating protein PM26 antibody; Q-snare antibody; RGD1306660 antibody; SLT1 antibody; SNARE-like tail-anchored protein 1 homolog antibody; Unconventional SNARE in the ER 1 homolog (S. cerevisiae) antibody; Unconventional SNARE in the ER 1 homolog antibody; Use1 antibody; USE1 like protein antibody; Use1 unconventional SNARE in the ER 1 homolog (S. cerevisiae) antibody; USE1-like protein antibody; USE1_HUMAN antibody; USE1L antibody; Vesicle transport protein USE1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Vesicle transport protein USE1 protein (1-146AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The USE1 Antibody (Product code: CSB-PA889157LA01HU) is Non-conjugated. For USE1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA889157LB01HU	USE1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA889157LC01HU	USE1 Antibody, FITC conjugated
Biotin	CSB-PA889157LD01HU	USE1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:500
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.

Gene References into Functions

Grsf1 controls translation of the protein Use1, possibly by positioning the 40S ribosomal subunit and associated translation factors in front of the translation start site. PMID: 25184340
FAT10 ubiquitination of USE1 leads to its accelerated degradation by the proteasome. PMID: 24528925
USE1 is not only the first E2 enzyme but also the first known substrate of FAT10 conjugation, as it was efficiently auto-FAT10ylated in cis but not in trans. PMID: 20975683
Results together suggest that NAG links between p31 and ZW10-RINT-1 and is involved in Golgi-to-ER transport. PMID: 19369418

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type IV membrane protein.

Protein Families

USE1 family

Database Links

HGNC: 30882

OMIM: 610675

KEGG: hsa:55850

STRING: 9606.ENSP00000263897

UniGene: Hs.16187

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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