E2-Tag Monoclonal Antibody

Derived from the transactivator protein of Bovine Papillomavirus type 1, the E2 tag is a compact peptide sequence composed of just 10 amino acids. This miniature yet highly immunogenic tag is frequently appended to recombinant constructs to facilitate straightforward protein detection and affinity purification. Its small size ensures that it imposes minimal steric hindrance, thereby preserving the native folding and biological function of the target protein.

The Mouse Anti-E2-Tag Monoclonal Antibody (CSB-MA000168, IgG isotype) is meticulously developed to offer monospecific and reliable recognition of this specific epitope. Generated through rigorous hybridoma technology utilizing a precisely synthesized peptide immunogen, this reagent guarantees exceptional specificity and robust lot-to-lot reproducibility.

Validated for both Enzyme-Linked Immunosorbent Assay (ELISA) and Western Blotting (WB) applications, this antibody exhibits outstanding sensitivity and high binding affinity. As vividly demonstrated in the accompanying Western Blot analysis, the antibody successfully detects 2 µg of the E2 fusion protein at impressively high working dilutions of 1:5,000 and 1:10,000. The presence of distinct, intensely staining bands at both concentrations—conspicuously devoid of background interference or non-specific artifacts—unequivocally confirms the antibody's superior immunoreactivity and excellent signal-to-noise ratio. Such robust performance at minimal concentrations translates into significant cost savings, making it a remarkably economical and powerful tool for high-throughput laboratory applications.

This monoclonal antibody is an indispensable asset for scientists engaged in molecular and cellular biology. It acts as a definitive reagent for verifying E2-tagged protein expression, accurately assessing molecular weight, and monitoring the efficiency of affinity purification protocols. With its proven reliability and versatility, the E2-Tag Monoclonal Antibody consistently delivers high-fidelity results, effectively supporting groundbreaking research in protein engineering, structural genomics, and related disciplines.