Flag-Tag Monoclonal Antibody

The FLAG tag is a widely used epitope tag that enables sensitive detection and affinity purification of recombinant fusion proteins in molecular and cell biology research. This Mouse IgG Flag-Tag monoclonal antibody (Code: CSB-MA000156) is generated against a synthetic peptide and supplied in a liquid, non-conjugated format, providing consistent recognition of FLAG-tagged recombinant proteins with high batch-to-batch reproducibility and reliable specificity. Stringent quality control supports dependable performance across multiple validated applications, making it suitable for routine protein expression analysis and immunoaffinity experiments. Experimental validation demonstrates strong immunofluorescence staining of FLAG-transfected HEK293 cells, with distinct red fluorescence detected using a 1:2000 antibody dilution, while DAPI counterstaining identifies cell nuclei. In immunoprecipitation assays, the antibody efficiently enriches FLAG-tagged fusion proteins from transfected HEK293 cell lysates, whereas normal mouse IgG and Protein G controls show no comparable enrichment. WB analysis further detects the FLAG fusion protein with clear, well-defined bands using antibody dilutions up to 1:5000, and recombinant FLAG-tagged protein expressed in E. coli is also specifically recognized. This reagent has been cited in two published studies, offering added confidence for researchers seeking proven, literature-supported tools for recombinant protein work. Validated applications include ELISA, WB, IF, and IP, supporting studies of FLAG-tagged recombinant protein expression, localization, purification, interaction analysis, and epitope tag-based protein characterization.