His-Tag Monoclonal Antibody

The His-Tag is one of the most universally adopted affinity tags in recombinant protein expression and purification. Its widespread use is attributed to the strong and specific coordination between the imidazole rings of histidine and immobilized transition metal ions (such as Ni²⁺ or Co²⁺). Beyond its primary role in affinity chromatography, the His-tag also serves as an efficient epitope for immunological detection.

The Mouse Anti-His-Tag Monoclonal Antibody (CSB-MA000300, IgG isotype) is specifically developed to provide high-affinity recognition of His-tagged proteins. Produced via hybridoma technology using a synthetic His-tag peptide as the immunogen, this antibody guarantees exceptional specificity, lot-to-lot consistency, and reliable performance in complex sample matrices.

Validated for both ELISA and Western Blot (WB) applications, the antibody exhibits outstanding sensitivity. The Western Blot analysis demonstrates its remarkable immunoreactivity, revealing crisp, distinct bands against both samples at a high working dilution of 1:10,000. Lane 1 shows a strong signal for the purified His-MBP recombinant protein, while Lane 2 confirms specific detection in His-Tag transfected 293T mammalian cells. This indicates the antibody's ability to recognize His-tagged proteins regardless of whether they are expressed in prokaryotic or eukaryotic systems. The clear signal retention even at this high dilution underscores the antibody's high titer and excellent signal-to-noise ratio, making it a highly cost-effective reagent for routine laboratory use.

This antibody is an indispensable tool for molecular biologists. It enables reliable confirmation of successful protein expression, accurate assessment of protein molecular weight, and serves as a critical reagent for evaluating the efficiency of affinity purification workflows.