| Code | CSB-MA000166 |
| Size | US$119 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:5000 |
Red fluorescent protein (RFP) is a widely utilized fluorescent reporter tag for tracking recombinant protein expression, subcellular localization, and purification in cellular and molecular biology research. This RFP-Tag monoclonal antibody (CSB-MA000166) is generated in mouse with IgG isotype, raised against recombinant RFP protein, and supplied as a non-conjugated liquid formulation dedicated to tag-based protein detection. It features high specificity and affinity for RFP-fused recombinant proteins, negligible non-specific background, and a broad working dilution window, ensuring consistent, cost-effective performance across routine experiments and high-throughput analytical workflows. WB validation reveals sharp, distinct target bands against 1 μg of RFP fusion protein at primary antibody dilutions of 1:3000, 1:5000 and 1:10000, with an optimized recommended dilution range of 1:500–1:5000, verifying superior sensitivity and target specificity with no detectable off-target signals. Fully validated for ELISA and WB applications, this tag antibody provides a dependable solution for the identification, expression quantification, and affinity purification tracking of RFP-tagged recombinant proteins in biochemical research, protein engineering, and biopharmaceutical development.
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