Product Details

Purity

>96% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 μg/ml, corresponding to a specific activity of >200 IU/mg.

Target Names

CXCL17

Uniprot No.

Q6UXB2

Research Area

Immunology

Alternative Names

CXCL17; VCC1; UNQ473/PRO842C-X-C motif chemokine 17; 6-Cys CXCL17; Dendritic cell and monocyte chemokine-like protein; DMC; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17]

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

22-119aa

Complete Sequence

SSLNPGVARG HRDRGQASRR WLQEGGQECE CKDWFLRAPR RKFMTVSGLP KKQCPCDHFK GNVKKTRHQR HHRKPNKHSR ACQQFLKQCQ LRSFALPL

Mol. Weight

11.5 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 3 % Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CXCL17, also known as VEGF coregulated chemokine 1, plays a distinctive role in mucosal immunity and angiogenesis-linked chemotaxis, making it a valuable tool for dissecting crosstalk between immune cell recruitment and vascular signaling. This tag-free recombinant human CXCL17 encompasses the full-length mature sequence (22–119 aa) expressed in *E. coli* and demonstrates an ED50 of less than 5.0 μg/ml for inducing VEGF expression in murine endothelial cells, corresponding to a specific activity exceeding 200 IU/mg — data that supports use in chemotaxis and migration assays, VEGF-dependent signaling pathway studies, and cell-based functional assays examining endothelial activation or macrophage recruitment. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts in sensitive immune cell experiments and in vivo inflammation models, while purity above 96% by SDS-PAGE provides a suitable basis for deployment as a reference standard in cytokine detection assays or as a reliable antigen for antibody development and ELISA validation.

Target Background

Function

Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells. Plays a role in angiogenesis and possibly in the development of tumors. Acts as an anti-inflammatory in the stomach. May play a role in the innate defense against infections. Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions.; seems exhibit much higher chemoattractant potency on monocytes and macrophages than 6-Cys CXCL17.

Gene References into Functions

High Lymph node CXCL17 messenger RNA is associated with colon cancer. PMID: 30198422
High expression of CXCL17 correlates with shorter overall survival of breast cancer patients.CXCL17 interacts with CXCR8 in breast cancer cells. PMID: 28943434
CXCL17 attenuates Imiquimod -induced psoriasis-like skin inflammation by recruiting myeloid-derived suppressor cells and regulatory T cells , which may be important for regulating excessive inflammation in psoriasis skin. PMID: 28389593
This article shows that GPR35 is the receptor of CXCL17. PMID: 25411203
CXCL17 production correlated with adverse immune infiltration and might be an important target for anti-HCC therapies. PMID: 25303284
We conclude that CXCL17 is an antimicrobial mucosal chemokine that may play a role in the pathogenesis of interstitial lung diseases PMID: 22611239
these results suggest that VCC-1 is involved in cisplatin-induced apoptosis of HepG2 cells, and also provides some evidence for VCC-1 as a potential cellular target for chemotherapy. PMID: 22425983
An Fc-fusion protein of 35kDa with a modified human immunoglobulin IgG1 Fc domain is capable of inhibiting CC chemokine-induced calcium flux, chemotaxis, and beta-arrestin recruitment in primary macrophages and transfected cells. PMID: 21586597
Immune surveillance occurs during the early intraepithelial stages of human pancreatic carcinogenesis and is mediated by expression of CXCL17 and ICAM2. PMID: 20955708
DMC induces migration of monocytes and immature dendritic cells. Expression studies show that DMC is constitutively expressed in lung, suggesting a potential role for DMC in recruiting monocytes and dendritic cells from blood into lung parenchyma PMID: 16455961
VCC1 plays a role in angiogenesis and possibly in the development of tumors in some tissue types PMID: 16989774
These results strongly suggest that VCC-1 plays an important role in hepatocellular carcinoma cell invasion and tumor growth. PMID: 19657564

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Tissue Specificity

Detected in trachea, stomach, lung and skeletal muscle. Detected in intestine and in normal and asthmatic lung (at protein level). Breast tumors showed 3- to 24-fold up-regulation.

Database Links

HGNC: 19232

OMIM: 611387

KEGG: hsa:284340

STRING: 9606.ENSP00000472467

UniGene: Hs.445586

Code	CSB-AP000781HU
Abbreviation	Recombinant Human CXCL17 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human C-X-C motif chemokine 17 protein (CXCL17) (Active)

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