Human chemokine (C-X-C motif) ligand 17 (CXCL17) ELISA kit

Code CSB-EL006246HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
chemokine (C-X-C motif) ligand 17 (CXCL17)
Alternative Names
CXCL17 ELISA Kit; VCC1 ELISA Kit; UNQ473/PRO842C-X-C motif chemokine 17 ELISA Kit; 6-Cys CXCL17 ELISA Kit; Dendritic cell and monocyte chemokine-like protein ELISA Kit; DMC ELISA Kit; VEGF coregulated chemokine 1) [Cleaved into: 4-Cys CXCL17] ELISA Kit
Abbreviation
CXCL17
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range
125 pg/mL-8000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CXCL17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %82-94
1:2Average %90
Range %84-95
1:4Average %96
Range %92-100
1:8Average %93
Range %90-96
Recovery
The recovery of human CXCL17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-102
EDTA plasma (n=4)9890-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.174 2.199 2.187 2.022
40001.459 1.499 1.479 1.314
20000.999 0.973 0.986 0.821
10000.638 0.692 0.665 0.500
5000.450 0.468 0.459 0.294
2500.324 0.342 0.333 0.168
1250.247 0.259 0.253 0.088
00.164 0.166 0.165  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CXCL17 ELISA Kit was designed for the quantitative measurement of Human CXCL17 protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

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Target Background

Function
(From Uniprot)
Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells. Plays a role in angiogenesis and possibly in the development of tumors. Acts as an anti-inflammatory in the stomach. May play a role in the innate defense against infections. Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions.; seems exhibit much higher chemoattractant potency on monocytes and macrophages than 6-Cys CXCL17.
Gene References into Functions
  1. High Lymph node CXCL17 messenger RNA is associated with colon cancer. PMID: 30198422
  2. High expression of CXCL17 correlates with shorter overall survival of breast cancer patients.CXCL17 interacts with CXCR8 in breast cancer cells. PMID: 28943434
  3. CXCL17 attenuates Imiquimod -induced psoriasis-like skin inflammation by recruiting myeloid-derived suppressor cells and regulatory T cells , which may be important for regulating excessive inflammation in psoriasis skin. PMID: 28389593
  4. This article shows that GPR35 is the receptor of CXCL17. PMID: 25411203
  5. CXCL17 production correlated with adverse immune infiltration and might be an important target for anti-HCC therapies. PMID: 25303284
  6. We conclude that CXCL17 is an antimicrobial mucosal chemokine that may play a role in the pathogenesis of interstitial lung diseases PMID: 22611239
  7. these results suggest that VCC-1 is involved in cisplatin-induced apoptosis of HepG2 cells, and also provides some evidence for VCC-1 as a potential cellular target for chemotherapy. PMID: 22425983
  8. An Fc-fusion protein of 35kDa with a modified human immunoglobulin IgG1 Fc domain is capable of inhibiting CC chemokine-induced calcium flux, chemotaxis, and beta-arrestin recruitment in primary macrophages and transfected cells. PMID: 21586597
  9. Immune surveillance occurs during the early intraepithelial stages of human pancreatic carcinogenesis and is mediated by expression of CXCL17 and ICAM2. PMID: 20955708
  10. DMC induces migration of monocytes and immature dendritic cells. Expression studies show that DMC is constitutively expressed in lung, suggesting a potential role for DMC in recruiting monocytes and dendritic cells from blood into lung parenchyma PMID: 16455961
  11. VCC1 plays a role in angiogenesis and possibly in the development of tumors in some tissue types PMID: 16989774
  12. These results strongly suggest that VCC-1 plays an important role in hepatocellular carcinoma cell invasion and tumor growth. PMID: 19657564

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Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Tissue Specificity
Detected in trachea, stomach, lung and skeletal muscle. Detected in intestine and in normal and asthmatic lung (at protein level). Breast tumors showed 3- to 24-fold up-regulation.
Database Links

HGNC: 19232

OMIM: 611387

KEGG: hsa:284340

STRING: 9606.ENSP00000472467

UniGene: Hs.445586

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