Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration of 10-100 ng/ml.

Target Names

Ccl24

Uniprot No.

Q9JKC0

Research Area

Immunology

Alternative Names

Ccl24; Scya24; C-C motif chemokine 24; Eosinophil chemotactic protein 2; Eotaxin-2; Small-inducible cytokine A24

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

27-119aa

Complete Sequence

VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV

Mol. Weight

10.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL24 (eotaxin-2) is a potent CC chemokine that selectively recruits eosinophils and basophils via CCR3, making it a critical mediator in allergic inflammation and type 2 immune responses. This tag-free recombinant mouse CCL24, encompassing the full-length mature sequence (residues 27–119), demonstrates confirmed biological activity in murine lymphocyte chemotaxis bioassays at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, CCR3 signaling studies involving MAPK and calcium flux pathways, and in vivo models of airway or skin inflammation. The endotoxin level of less than 1.0 EU/μg satisfies the stringent criteria needed to prevent LPS-driven artifacts in sensitive immune cell functional assays, while purity exceeding 97% by SDS-PAGE supports its use as a reliable reference standard in cytokine detection ELISAs or as a positive control for anti-CCL24 antibody validation. Produced in *E. coli*, this preparation aligns with standards expected in quantitative receptor-ligand binding and desensitization experiments.

Target Background

Function

Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.

Gene References into Functions

the increased airway eosinophils and mucous cell metaplasia in house dust mite-challenged Cd163-/- mice are mediated by augmented CCL24 production PMID: 26376364
this study shows that TPL-2 inhibits Ccl24 expression in lung dendritic cells, and blockade of Ccl24 prevents the exaggerated airway eosinophilia and lung inflammation in mice given house dust mites-pulsed Map3k8-/- dendritic cells PMID: 27484038
production in M-CSF-induced bone marrow-derived macrophages is synergistically induced by IL-4 and IL-10 PMID: 25267883
Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
Data indicate that eotaxin 2 (CCL-24), which is known to influence eosinophil migration, was highly up-regulated in broncho-alveolar macrophages (BAMs). PMID: 23685352
The oesophageal production of CCL24 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
Results demonstrate a cooperative mechanism between interleukin-13 and eotaxin-2, where IL-13 mediates allergen-induced eotaxin-2 expression, and eotaxin-2 mediates IL-13-induced airway eosinophilia. PMID: 15647285
Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
The eotaxin-2 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
CCL24 seems important for recruitment of eosinophils into BAL. PMID: 18850374

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis.

Database Links

KEGG: mmu:56221

STRING: 10090.ENSMUSP00000004936

UniGene: Mm.31505

Code	CSB-AP001361MO
Abbreviation	Recombinant Mouse Ccl24 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Mouse C-C motif chemokine 24 protein (Ccl24) (Active)

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