Mouse Eotaxin 2 ELISA Kit

Instructions
Code CSB-E07278m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-C motif) ligand 24
Alternative Names Ccl24 ELISA Kit; Scya24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
Abbreviation CCL24
Uniprot No. Q9JKC0
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse eotaxin 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %98
Range %89-102
1:2Average %90
Range %82-95
1:4Average %91
Range %84-95
1:8Average %91
Range %85-94
Recovery
The recovery of mouse eotaxin 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10192-106
EDTA plasma (n=4)9184-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.612 2.442 2.527 2.420
52.199 2.111 2.155 2.048
2.51.453 1.487 1.470 1.363
1.250.752 0.734 0.743 0.636
0.6250.348 0.341 0.345 0.238
0.3130.242 0.249 0.246 0.139
0.1560.170 0.163 0.167 0.060
00.108 0.106 0.107
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Binds to CCR3 (By similarity).
Gene References into Functions
  1. the increased airway eosinophils and mucous cell metaplasia in house dust mite-challenged Cd163-/- mice are mediated by augmented CCL24 production PMID: 26376364
  2. this study shows that TPL-2 inhibits Ccl24 expression in lung dendritic cells, and blockade of Ccl24 prevents the exaggerated airway eosinophilia and lung inflammation in mice given house dust mites-pulsed Map3k8-/- dendritic cells PMID: 27484038
  3. production in M-CSF-induced bone marrow-derived macrophages is synergistically induced by IL-4 and IL-10 PMID: 25267883
  4. Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
  5. Data indicate that eotaxin 2 (CCL-24), which is known to influence eosinophil migration, was highly up-regulated in broncho-alveolar macrophages (BAMs). PMID: 23685352
  6. The oesophageal production of CCL24 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
  7. Results demonstrate a cooperative mechanism between interleukin-13 and eotaxin-2, where IL-13 mediates allergen-induced eotaxin-2 expression, and eotaxin-2 mediates IL-13-induced airway eosinophilia. PMID: 15647285
  8. Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
  9. The eotaxin-2 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
  10. eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
  11. CCL24 seems important for recruitment of eosinophils into BAL. PMID: 18850374

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Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Tissue Specificity Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis.
Database Links

KEGG: mmu:56221

STRING: 10090.ENSMUSP00000004936

UniGene: Mm.31505

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