Recombinant Mouse C-C motif chemokine 6 protein (Ccl6) (Active)

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Code CSB-AP001251MO
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Size $354
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Product Details

Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml.
Target Names
Ccl6
Uniprot No.
Research Area
Immunology
Alternative Names
Ccl6; C10; Scya6C-C motif chemokine 6; Protein C10; Small-inducible cytokine A6) [Cleaved into: CCL6(22-95); CCL6(23-95)]
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
22-116aa
Complete Sequence
GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA
Mol. Weight
10.7 kDa
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
CCL6(22-95) and CCL6(23-95) are potent chemoattractants.
Gene References into Functions
  1. Mice lacking RXRalpha in myeloid cells exhibit reduced levels of CCL6 and CCL9, impaired recruitment of leukocytes to sites of inflammation, and lower susceptibility to sepsis. PMID: 20498053
  2. enhanced expression in intestinal epithelium following immunostimulation with LPS or pro-inflammatory cytokines PMID: 19812544
  3. the injury-induced expression of C10 at the mRNA and protein level PMID: 11895334
  4. c10 promotes fibrosis by recruiting macrophages in the inflammatory response to bleomycin in the lung PMID: 12356575
  5. CCL6 gene activation and production induced by SCF in the presence or absence of rather specific inhibitors for certain pathways demonstrated that the MEK/MAPK pathway but not the PI-3K pathway was crucial for the SCF-induced CCL6 gene activation. PMID: 17234680
  6. the chemokines CCL6 and CCL9 as genes prominently induced by the type I IFNs and ICSBP PMID: 19171873

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Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Tissue Specificity
Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.
Database Links

KEGG: mmu:20305

STRING: 10090.ENSMUSP00000019071

UniGene: Mm.137

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