Recombinant Mouse C-C motif chemokine 6 protein (Ccl6)

CCL6 (C10) is a murine CC chemokine that signals through CCR1 to regulate monocyte and macrophage recruitment during inflammatory and infectious responses. This tag-free recombinant protein encompasses the full-length mature sequence (residues 22–116), and its biological activity has been confirmed via chemotaxis bioassay using human CCR1-transfected BaF3 cells at an effective concentration range of 10–100 ng/mL, providing a suitable basis for chemotaxis and migration assays, downstream CCR1 signaling studies involving MAPK and NF-κB pathway activation, and in vivo mouse models of inflammation or infection. Endotoxin levels below 1.0 EU/μg minimize the risk of LPS-driven artifacts in sensitive immune cell functional assays, while purity exceeding 97% by SDS-PAGE supports use as a reliable reference standard in cytokine detection assays or as a positive control for antibody validation by ELISA and Western blot. Produced in *E. coli*, this recombinant CCL6 protein aligns with standards expected in rigorous cell-based and in vivo immunology research.