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Recombinant Rat CXC chemokine RTCK1 protein (Ppbp), partial (Active)

Recombinant Rat CXC chemokine RTCK1 protein (Ppbp), partial (Active)

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Code	CSB-AP001441RA
Abbreviation	Recombinant Rat Ppbp protein, partial (Active)
MSDS	MSDS Datasheet
Size	$354
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Product Details
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Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.1-1.0 ng/ml.

Target Names

Ppbp

Uniprot No.

Q99ME0

Research Area

Immunology

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

46-107aa

Complete Sequence

IELRCRCTNT LSGIPLNSIS RVNVFRPGAH CDNVEVIATL KNGKEVCLDP TAPMIKKIVK KI

Mol. Weight

6.8 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CXCL7 (RTCK1) drives neutrophil chemotaxis through CXCR2 signaling, making it a key mediator in inflammatory recruitment cascades. This tag-free recombinant rat Ppbp fragment (residues 46–107) represents the mature, processed form of the protein and demonstrates potent chemotactic activity at 0.1–1.0 ng/ml in a CXCR2-transfected BaF3 cell migration assay, providing a suitable basis for chemotaxis and migration studies, CXCR2-dependent MAPK and NF-κB signaling pathway dissection, and in vivo rat inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound immune cell functional readouts, which supports use in sensitive neutrophil activation assays and as a reference standard in cytokine detection platforms. Produced in *E. coli* with >97% purity by SDS-PAGE, this protein also meets the quality thresholds commonly required for antibody development workflows including immunization, ELISA coating, and Western blot positive controls.