ADY2 Antibody

Code CSB-PA333089XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ADY2 Polyclonal antibody
Uniprot No.
Target Names
ADY2
Alternative Names
ADY2; ATO1; YCR010C; YCR10C; Accumulation of dyads protein 2; Ammonia transport outward protein 1
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ADY2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transporter protein required for ammonia export and acetate uptake and resistance. Necessary for up-regulation and down-regulation of meiotic plaque (MP) component levels in a dependency on external acetate. Has a role in ascus formation.
Gene References into Functions
  1. deletion of SNF1 abolishes the increase of histone H3 acetylation at the promoter of the glucose-repressed ADY2 gene, and as a consequence profoundly affects the chromatin structural alterations accompanying transcriptional activation PMID: 22306658
  2. there is an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p PMID: 22579979
  3. study demonstrated that monocarboxylate transporters Jen1 and Ady2 are modulators of lactic acid production PMID: 22260735
  4. The authors show that neighbouring microcolonies coordinate production of Ato1p-green fluorescent protein. PMID: 19302539
  5. These data suggest that besides the ammonia induction of Ato protein synthesis, pH may rapidly regulate Ato1p function. PMID: 17395151

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note=Localizes to large detergent resistant patches of the cell membrane (DRM) enriched in ergosterol and sphingolipids (PubMed:17395151).
Protein Families
Acetate uptake transporter (AceTr) (TC 2.A.96) family
Database Links

KEGG: sce:YCR010C

STRING: 4932.YCR010C

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