ALA3 Antibody

Code CSB-PA896398XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ALA3 Polyclonal antibody
Uniprot No.
Target Names
ALA3
Alternative Names
ALA3 antibody; ITB2 antibody; At1g59820 antibody; F23H11.14Phospholipid-transporting ATPase 3 antibody; AtALA3 antibody; EC 7.6.2.1 antibody; Aminophospholipid ATPase 3 antibody; Aminophospholipid flippase 3 antibody; Protein IRREGULAR TRICHOME BRANCH 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ALA3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in transport of phospholipids. Contributes to transmembrane flipping of lipids. Required for secretory processes during plant development. Requires an interaction with an ALIS protein for activity. Has activity with phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine, but not with lysolipid. Modifies endomembranes in multiple cell types, enabling structural changes, or signaling functions that are critical for normal development and adaptation to varied growth environments. Required for the trafficking and endocytic recycling of ABCG36/PEN3 between the trans-Golgi network and the plasma membrane; thus promoting ABCG36/PEN3 recruitment to the host-pathogen interface upon infection by powdery mildews (e.g. Blumeria graminis) and bacteria (e.g. Pseudomonas syringae), or upon the detection of pathogen-associated molecular patterns (PAMPs) (e.g. flg22 and chitin).
Gene References into Functions
  1. ALA3 functions to modify endomembranes in multiple cell types PMID: 23667493
  2. Data show that ALA3 localizes to the Golgi apparatus and that mutations of ALA3 result in impaired growth of roots and shoots. PMID: 18344284
  3. Mutations in ALA3 reduce trichome branch lengths, distort trichome shape, reduce primary root growth and increase root hair length. PMID: 19566596
Subcellular Location
Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IV subfamily
Tissue Specificity
Expressed in petals and sepals, but not in reproductive tissues. In siliques, detected in the upper part of the seed pod and in the area between the seed pod and the stem, but not in developing seeds. Strong expression in vascular shoot tissues and in sto
Database Links

KEGG: ath:AT1G59820

STRING: 3702.AT1G59820.1

UniGene: At.36777

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