Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ALMT1 Polyclonal antibody

Uniprot No.

Q9SJE9

Target Names

ALMT1

Alternative Names

ALMT1; At1g08430; T27G7.11; Aluminum-activated malate transporter 1; AtALMT1

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ALMT1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Malate transporter critical for aluminum tolerance. The STOP1 transcription factor is required for ALMT1 expression.

Gene References into Functions

Results indicate STOP1 and ALMT1 as a signalling pathway of low phosphate (Pi) availability and exuded malate as an unexpected apoplastic inhibitor of root cell wall expansion. PMID: 28504266
CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR2 to be an activator of AtALMT1 expression. PMID: 25627216
The exudation of malic acid from roots in response to Ni treatment in either susceptible or tolerant plant species was found to be partially dependent on AtALMT1 expression. PMID: 22411507
Expression of AtALMT1(P)::AtMATE not only significantly increased Al resistance of the transgenic plants, but also enhanced carbon-use efficiency for Al resistance. PMID: 22413742
overexpression of AtALMT1 can be used as an approach to enhance a plant's ability to release malate into the rhizosphere. PMID: 23857348
WRKY46 functions in Al sensitivity through regulating the expression of ALMT1. WRKY46 represses ALMT1 expression and malate secretion. PMID: 24118304
the complex regulation of AtALMT1 expression in response to phytohormones shows the change in AtALMT1 expression during the adaptation to abiotic and biotic stresses PMID: 23624855
AtALMT1 is an essential factor for Aluminum tolerance in Arabidopsis but does not represent the major aluminum tolerance QTL also found on chromosome 1 [AtALMT1] PMID: 16740662
This study characterized AtALMT1, an Arabidopsis aluminum-activated malate transporter, to clarify its specific role in malate release and aluminum stress responses. PMID: 17885092
AtALMT1 functions independently from AtMATE to confer aluminum tolerance. PMID: 18826429

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Aromatic acid exporter (TC 2.A.85) family

Tissue Specificity

Expressed in roots, but not in shoots. Detected in the root apex in absence of aluminum stress and in root apices, the stele and endodermis of the elongating zone of primary and lateral roots after aluminum stress. Not expressed in cortical and epidermal

Database Links

KEGG: ath:AT1G08430

STRING: 3702.AT1G08430.1

UniGene: At.42261

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ALMT1 Antibody

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