ARF1 Antibody

Code CSB-PA001988XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ARF1 Polyclonal antibody
Uniprot No.
Target Names
ARF1
Alternative Names
ARF1 antibody; YDL192W antibody; D1244ADP-ribosylation factor 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ARF1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Recruits polyadenylate-binding protein PAB1 to COPI vesicles, and this is required for correct localization of the asymmetrically distributed ASH1 mRNA.
Gene References into Functions
  1. Arf1 and the exomer cargo adaptor coordinate cargo sorting into subset of secretory vesicle carriers in the model organism Saccharomyces cerevisiae. Here, we detail the assays we used to explore the cooperative action of Arf1 and exomer to bind and remodel membranes. PMID: 27632000
  2. The DCB/HUS domain amplifies the ability of Sec7 to activate Arf1 on the membrane surface by facilitating membrane insertion of the Arf1 amphipathic helix. PMID: 26765562
  3. Arf1 is involved in mitochondrial homeostasis and dynamics, independent of its role in vesicular traffic. PMID: 25190516
  4. Study demonstrates V-ATPase interacts with two distinct GTPases, Arf1 and Gtr1, which are required for Ras and TORC1 activation, respectively. PMID: 25002144
  5. Study determined the structure of Arf1 bound to the gammazeta-COP subcomplex of coatomer; and finds that betadelta/gammazeta-COP interacts with two molecules of Arf1-GTP, which bind to quasi-equivalent sites on the large beta-COP and gamma-COP subunits. PMID: 22304919
  6. nucleosome shielding is the primary means to maintain promoter fidelity, and histone replacement is most efficiently mediated in yeast cells by a HIR/Asf1/H3-H4/Rtt106 pathway. PMID: 22128187
  7. Here we demonstrate that Trs65p directly binds to the C-terminus of the Arf1p exchange factor Gea2p and provide in vivo evidence that this interaction is physiologically relevant PMID: 21813735
  8. Arf1p has a role in linking COPI vesicles and mRNA in Saccharomyces cerevisiae PMID: 15356266
  9. Arf1p and Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi PMID: 15504911
  10. COPI in these mutants is released from Golgi membranes by brefeldin A, a drug that binds directly to guanine nucleotide exchange factor for ADP ribosylation factors and blocks Arf1 activation PMID: 15930122
  11. ScARF1 and ScARF3 are the yeast orthologs of mammalian ARF1 and ARF6 and have roles in the control of peroxisome proliferation PMID: 16100119

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Subcellular Location
Golgi apparatus.
Protein Families
Small GTPase superfamily, Arf family
Database Links

KEGG: sce:YDL192W

STRING: 4932.YDL192W

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