ARF3 Antibody

Code CSB-PA001990XA01DOA
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Product Details

Full Product Name Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF3 Polyclonal antibody
Uniprot No. P40940
Target Names ARF3
Alternative Names ARF3 antibody; ARL1 antibody; At2g24765 antibody; F27A10.8 antibody; F27C12.31 antibody; ADP-ribosylation factor 3 antibody; AtARF3 antibody; Protein ARF-LIKE 1 antibody; AtARL1 antibody
Raised in Rabbit
Species Reactivity Arabidopsis thaliana
Immunogen Recombinant Arabidopsis thaliana ARF3 protein
Immunogen Species Arabidopsis thaliana (Mouse-ear cress)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
Gene References into Functions
  1. AS2, RH10 and NUC1 maintain cytosine methylations at specific sets of CpG sites in exon 6 of ARF3. PMID: 29415182
  2. Results indicate that auxin response factors AtARF2-AtARF4 and AtARF5 play significant roles in regulating both female and male gametophyte development. PMID: 29145642
  3. Analysis of ta-siRNA synthesis mutants and mutated ARF3-overexpressing plants that escape the tasiRNA-ARF target indicated that self-pollination was hampered by short stamens in plants under drought and high-salinity stress. PMID: 25243128
  4. ARF3 is a direct target of AP2 and partially mediates AP2's function in floral meristem determinacy.ARF3 integrates the functions of AGAMOUS and APETALA2 in floral meristem determinacy. PMID: 25187180
  5. ARF3 mediates the auxin response during de novo shoot regeneration. PMID: 23124326
  6. AS1 binds the ETT promoter. An upstream region of ETT directs transcriptional repression by AS1 and AS2. AS2 requires AS1 to regulate ETT expression directly. PMID: 23571218
  7. ARL1(AT2G24765) is associated with the localization of peripheral golgins to the plant Golgi apparatus. PMID: 16830178

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Subcellular Location Golgi apparatus.
Protein Families Small GTPase superfamily, Arf family
Database Links

KEGG: ath:AT2G24765

STRING: 3702.AT2G24765.1

UniGene: At.166

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