ARF1 Antibody

Code CSB-PA810539XA01DOA
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Product Details

Full Product Name Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF1 Polyclonal antibody
Uniprot No. Q8L7G0
Target Names ARF1
Alternative Names ARF1 antibody; At1g59750 antibody; F23H11.7Auxin response factor 1 antibody
Raised in Rabbit
Species Reactivity Arabidopsis thaliana
Immunogen Recombinant Arabidopsis thaliana ARF1 protein
Immunogen Species Arabidopsis thaliana (Mouse-ear cress)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Acts as repressor of IAA2, IAA3 and IAA7.
Gene References into Functions
  1. The authors showed that Arf1 interacted with the viral p27 replication protein within the virus-induced large punctate structures of the endoplasmic reticulum membrane. PMID: 23097452
  2. unlike arf2 mutations, an arf1 mutation increased transcription of Aux/IAA genes in Arabidopsis flowers, supporting previous biochemical studies that indicated that ARF1 is a transcriptional repressor PMID: 16176952
  3. The half-life of degradation of ARF1, and its role in auxin metabolism, are reported. PMID: 18088308
Subcellular Location Nucleus.
Protein Families ARF family
Tissue Specificity Expressed in the whole plant.
Database Links

KEGG: ath:AT1G59750

STRING: 3702.AT1G59750.1

UniGene: At.74832

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