ARF3 Antibody

Code CSB-PA515815XA01DOA
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Product Details

Full Product Name Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF3 Polyclonal antibody
Uniprot No. O23661
Target Names ARF3
Alternative Names ARF3 antibody; ETT antibody; At2g33860 antibody; T1B8.30Auxin response factor 3 antibody; Protein ETTIN antibody
Raised in Rabbit
Species Reactivity Arabidopsis thaliana
Immunogen Recombinant Arabidopsis thaliana ARF3 protein
Immunogen Species Arabidopsis thaliana (Mouse-ear cress)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Involved in the establishment or elaboration of tissue patterning during gynoecial development.
Gene References into Functions
  1. ETT controls developmental processes in the Arabidopsis shoot through its auxin-sensing property. PMID: 28804059
  2. Auxin Response Factor 3 (ARF3) was identified as a mediator of cross-talk between self-incompatibility signaling and pistil development. PMID: 23129621
  3. ATS and ETT were found to share an overlapping expression pattern during Arabidopsis ovule development and loss of either gene resulted in congenital fusion of the integuments and altered seed morphology. PMID: 22296848
  4. Mutations in ETTIN were isolated as second site suppressors and found to ameliorate ectopic KANADI activity throughout the plant as well. Mutant phenotypes of ett are restricted to flowers. PMID: 16199616
  5. Mutations delay the expression of adult traits, demonstrating that these genes have non-redundant roles in shoot maturation. PMID: 16818444

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Subcellular Location Nucleus.
Protein Families ARF family
Tissue Specificity Expressed in the whole plant.
Database Links

KEGG: ath:AT2G33860

STRING: 3702.AT2G33860.1

UniGene: At.10360

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