bfr Antibody

Code CSB-PA364636XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) bfr Polyclonal antibody
Uniprot No.
Target Names
bfr
Alternative Names
bfr antibody; b3336 antibody; JW3298Bacterioferritin antibody; BFR antibody; EC 1.16.3.1 antibody; Cytochrome b-1 antibody; Cytochrome b-557 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) bfr protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.
Gene References into Functions
  1. these findings confirm that even a single charge-inversion mutation at the 2-fold interface of BFR can affect the quaternary structure of its dimers and their ability to self-assemble into cage structures. PMID: 26115686
  2. Three computationally screened bioferritins are generated that present characterization of their stability and self assembly. PMID: 21488690
  3. a large proportion of BFR core iron is released in the absence of a high affinity Fe(2+) chelator and further that chelators are not passive participants in iron release reactions PMID: 21106523
  4. analysis of residues required for core formation in bacterioferritin PMID: 19439409
  5. the fluorescence intensity, due to the presence of two tryptophan residues (Trp35 and Trp133) in each of the 24 subunits, is highly sensitive to the iron status of the ferroxidase center and is quenched to different extents by Fe2+ and Fe3+ PMID: 19705876

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Protein Families
Bacterioferritin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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