Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BLM10 Polyclonal antibody

Uniprot No.

P43583

Target Names

BLM10

Alternative Names

BLM10 antibody; BLM3 antibody; YFL007W antibody; YFL006WProteasome activator BLM10 antibody; Bleomycin resistance protein BLM10 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BLM10 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Involved in DNA damage response in somatic cells: binds to acetylated histones and promotes degradation of histones following DNA double-strand breaks.

Gene References into Functions

data are consistent with a model in which Blm10 proteasome-mediated turnover of Dnm1 is required for the maintenance of mitochondrial function PMID: 24604417
Blm10 represents the first proteolytic core particle-dedicated nuclear import receptor in yeast. PMID: 23982732
Data propose that the cellular function of Blm10 is based on central core of the proteasome association and activation to promote the degradation of proteasome substrates. PMID: 22025621
Blm10 functions in a proteasome-related process and it is required for the maintenance of mitochondrial function. PMID: 20227375
Synthetic interactions between blm10Delta and other mutations that impair proteasome function show that Blm10 functions together with proteasomes in vivo PMID: 15778719
Data show that Blm10 binds in a defined orientation to both ends of the 20 S particle and contacts all the proteasome alpha subunits. PMID: 16952374
Blm10 and the 19 S activator with Pre4 have a partially redundant function in stabilizing nascent 20 S proteasomes and in promoting their activation PMID: 17911101
Blm10 distinguishes between gate conformations and regulates the activation of core particles. PMID: 18927584

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

BLM10 family

Database Links

KEGG: sce:YFL007W

STRING: 4932.YFL007W

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BLM10 Antibody

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Target Background