CAF4 Antibody

Code CSB-PA336133XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CAF4 Polyclonal antibody
Uniprot No.
Target Names
CAF4
Alternative Names
CAF4 antibody; YKR036CCCR4-associated factor 4 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CAF4 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in mitochondrial fission. Has a partially redundant function to MDV1 in acting as an adapter protein, binding to FIS1 on the mitochondrial outer membrane and recruiting the dynamin-like GTPase DNM1 to form mitochondrial fission complexes. Plays a key role in determining the polarized localization of those DNM1 clusters that are not immediately involved in the mitochondrial fission process.
Gene References into Functions
  1. Mdv1p and Caf4p are molecular adaptors that recruit Dnm1p to mitochondrial fission sites. PMID: 16009724
Subcellular Location
Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note=Uniformly distributed on the cytoplasmic face of the mitochondrial outer membrane. This localization is dependent on FIS1. Reorganizes to punctate structures on mitochondria, corresponding to mitochondrial constriction sites, at a late step in mitochondrial division.
Protein Families
WD repeat MDV1/CAF4 family
Database Links

KEGG: sce:YKR036C

STRING: 4932.YKR036C

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