CHK1 Antibody

Code CSB-PA005338XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CHK1 Polyclonal antibody
Uniprot No.
Target Names
CHK1
Alternative Names
CHK1 antibody; YBR274W antibody; YBR1742Serine/threonine-protein kinase CHK1 antibody; EC 2.7.11.1 antibody; Checkpoint kinase 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) CHK1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit.
Gene References into Functions
  1. Chk1 has a redundant role in the control of DIC or that DIC is redundant for maintaining genome stability PMID: 25551702
  2. Chk1p has a role in the intra-S-phase checkpoint activated when cells replicate their DNA PMID: 15229282
  3. CHK1 mutants are not defective in double-strand break repair-initiated sister chromatid exchange, after exposure to DNA damaging agents. PMID: 16039914
  4. The observations in the chk1Delta/chk1Delta mutant strain suggest that constitutive nonhomologous end-joining is involved in the repair of BLM-induced DSBs. PMID: 17624540
Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily
Database Links

KEGG: sce:YBR274W

STRING: 4932.YBR274W

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