CHL Antibody

Code CSB-PA447489XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CHL Polyclonal antibody
Uniprot No.
Target Names
CHL
Alternative Names
CHL antibody; At3g47860 antibody; T23J7.190Chloroplastic lipocalin antibody; AtCHL antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CHL protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Lipocalin that prevents thylakoidal membrane lipids peroxidation and confers protection against oxidative stress, especially mediated by singlet oxygen in response to high light and other stress (e.g. heat shocks). Required for seed longevity by insuring polyunsaturated lipids integrity.
Gene References into Functions
  1. AtTIL and AtCHL have overlapping functions in lipid protection which are essential for stress resistance and survival. PMID: 23837879
  2. AtCHL is localized in the thylakoid lumen, alleviates membrane lipid peroxidation, and increases tolerance towards oxidative stress induced by paraquat or drought treatment. PMID: 19674405
Subcellular Location
Plastid, chloroplast thylakoid lumen.
Protein Families
Calycin superfamily, Lipocalin family
Tissue Specificity
Expressed in leaves at low levels (at protein levels). Present in seeds.
Database Links

KEGG: ath:AT3G47860

STRING: 3702.AT3G47860.1

UniGene: At.3254

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