CKX1 Antibody

Code CSB-PA890499XA01ZAX
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Product Details

Full Product Name
Rabbit anti-Zea mays (Maize) CKX1 Polyclonal antibody
Uniprot No.
Target Names
CKX1
Alternative Names
CKX1Cytokinin dehydrogenase 1 antibody; EC 1.5.99.12 antibody; Cytokinin oxidase 1 antibody; CKO 1 antibody; COX 1 antibody; ZmCKX1 antibody
Raised in
Rabbit
Species Reactivity
Zea mays (Maize)
Immunogen
Recombinant Zea mays (Maize) CKX1 protein
Immunogen Species
Zea mays (Maize)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.
Gene References into Functions
  1. The high resolution X-ray structures of CKO1 reveal E381 as a key residue for enzyme specificity that restricts substrate binding as well as quinone electron acceptor binding. PMID: 26519657
  2. oxidation of hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one and coniferron generates transitional free radicals that are used by CKX as effective electron acceptors. PMID: 19912568
  3. CKX1 was present in the apoplast, whereas CKX10 was detected in the cytosol. PMID: 19436049
Subcellular Location
Secreted, extracellular space.
Protein Families
Oxygen-dependent FAD-linked oxidoreductase family
Tissue Specificity
Expressed in immature kernels and unpollinated cobs. Weakly expressed in kernels harvested two weeks after anthesis.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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