CKX1 Antibody

Code CSB-PA890499XA01ZAX
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Product Details

Full Product Name
Rabbit anti-Zea mays (Maize) CKX1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
CKX1Cytokinin dehydrogenase 1 antibody; EC antibody; Cytokinin oxidase 1 antibody; CKO 1 antibody; COX 1 antibody; ZmCKX1 antibody
Raised in
Species Reactivity
Zea mays (Maize)
Recombinant Zea mays (Maize) CKX1 protein
Immunogen Species
Zea mays (Maize)
Purification Method
Antigen Affinity Purified
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.
Gene References into Functions
  1. The high resolution X-ray structures of CKO1 reveal E381 as a key residue for enzyme specificity that restricts substrate binding as well as quinone electron acceptor binding. PMID: 26519657
  2. oxidation of hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one and coniferron generates transitional free radicals that are used by CKX as effective electron acceptors. PMID: 19912568
  3. CKX1 was present in the apoplast, whereas CKX10 was detected in the cytosol. PMID: 19436049
Subcellular Location
Secreted, extracellular space.
Protein Families
Oxygen-dependent FAD-linked oxidoreductase family
Tissue Specificity
Expressed in immature kernels and unpollinated cobs. Weakly expressed in kernels harvested two weeks after anthesis.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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