Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) chb Polyclonal antibody

Uniprot No.

Q9NBD7

Target Names

chb

Alternative Names

chb antibody; CLASP antibody; Mast antibody; MESR7 antibody; orbit antibody; CG32435CLIP-associating protein antibody; Misexpression suppressor of ras 7 antibody; Protein Multiple asters antibody; Mast antibody; Protein Orbit antibody; Protein chromosome bows antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) chb protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for several aspects of mitotic spindle formation including the formation of the overlapping central spindle microtubules and kinetochore attachment. Required for the incorporation of tubulin subunits at the plus ends of kinetochore microtubules during poleward microtubule flux. Acts antagonistically to Klp10A and Klp67A to maintain metaphase spindle length. Also required for guidance of CNS axons downstream of Abl. May function to identify a subset of microtubules that probe the peripheral growth cone domain, where guidance signals exert their influence on cytoskeletal organization. Also required during oogenesis for the organization of the polarized microtubule network inside the 16-cell cyst that ensures oocyte differentiation.

Gene References into Functions

Orbit plays an essential role as a connector between microtubules and the contractile ring in Drosophila male meiosis. PMID: 24850412
Orbit is involved in the proper orientation of spindles towards fusomes during synchronous mitosis of spermatogonial cysts. PMID: 23520495
MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity. PMID: 12034769
Data show that the Orbit/Mast microtubule-associated protein is required at several stages in Drosophila oocyte cell differentiation. PMID: 12538517
Orbit/Mast/CLASP localizes to interior rather than peripheral central spindle microtubules PMID: 15240569
is an essential component for microtubule subunit incorporation into fluxing K-fibres PMID: 15592460
results suggest that Mast plays an essential role in reducing the dynamic behaviour of microtubules by specifically promoting the pause state PMID: 17487886
Dynein and mast/orbit/CLASP have antagonistic roles in regulating kinetochore-microtubule plus-end dynamics. PMID: 19549687

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Subcellular Location

Cytoplasm, cytoskeleton. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cell projection, growth cone. Cleavage furrow. Note=Localizes to punctate cytoplasmic foci in interphase. Concentrates on spindle microtubules from prophase to late anaphase. Accumulates on the mid-part of the central spindle during telophase where it remains until cleavage. Subsequently appears at the periphery of the reforming karyomeres. Also localizes to the spindle remnant, cleavage furrows and the fusome of the 16-cell ovarian cyst. Localizes to the axonal growth cone within neurons.

Protein Families

CLASP family

Tissue Specificity

Expressed in testis and ovary.

Database Links

KEGG: dme:Dmel_CG32435

STRING: 7227.FBpp0088469

UniGene: Dm.1413

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chb Antibody

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