Sec5 Antibody

Code CSB-PA007878XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Sec5 Polyclonal antibody
Uniprot No.
Target Names
Sec5
Alternative Names
Sec5 antibody; CG8843Exocyst complex component 2 antibody; Exocyst complex component Sec5 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Sec5 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Gene References into Functions
  1. Sec5 concentrates at the sub-apical complex, indicating a role for Sec5 in the polarized epithelium. PMID: 20630948
  2. Sec5 differentiates between two forms of vesicle trafficking: trafficking for cell growth and membrane protein insertion depend on sec5, whereas transmitter secretion does not PMID: 12575951
  3. Germline clones of sec5 possess defects in membrane addition and the posterior positioning of the oocyte. PMID: 14681190
  4. Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function PMID: 15728258
  5. Rab11 forms a complex with Sec5. Sec5 interacts with Sec6 suggesting the exocyst is a Rab11 effector that facilitates protein transport to the apical rhabdomere in Drosophila photoreceptors PMID: 15897260
  6. The Drosophila exocyst component sec5 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. PMID: 16224820

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Protein Families
SEC5 family
Database Links

KEGG: dme:Dmel_CG8843

STRING: 7227.FBpp0077208

UniGene: Dm.288

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