fep1 Antibody

Datasheet

Code	CSB-PA611916XA01SXV
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Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) fep1 Polyclonal antibody

Uniprot No.

Q10134

Target Names

fep1

Alternative Names

fep1 antibody; gaf2 antibody; SPAC23E2.01Iron-sensing transcription factor 1 antibody; Transcription factor gaf2 antibody; Gaf-2 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) fep1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Transcriptional repressor that binds the consensus promoter sequence 5'-[AT]GATAA-3' during iron-replete conditions to downregulate transcription of target genes. Represses the expression of the iron transporter fio1 in response to high iron concentrations. Also represses the expression of str1, str2 and str3. Represses the expression of shu1 in presence of iron.

Gene References into Functions

Results describe the first example of a monothiol glutaredoxin (Grx4)that acts as an inhibitory partner for an iron-regulated transcription factor (fep1)under conditions of low iron levels. PMID: 21421748
Fep1 is a non-canonical GATA factor required for iron homeostasis PMID: 15866870
The N-terminal region of Fep1 is able to activate its transcription in response to iron. PMID: 19252122

Subcellular Location

Nucleus.

Database Links

KEGG: spo:SPAC23E2.01

STRING: 4896.SPAC23E2.01.1

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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fep1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background