GAL10 Antibody

Code CSB-PA356249XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAL10 Polyclonal antibody
Uniprot No.
Target Names
GAL10
Alternative Names
GAL10 antibody; YBR019C antibody; YBR0301Bifunctional protein GAL10 [Includes: UDP-glucose 4-epimerase antibody; EC 5.1.3.2 antibody; Galactowaldenase); Aldose 1-epimerase antibody; EC 5.1.3.3 antibody; Galactose mutarotase)] antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAL10 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.
Gene References into Functions
  1. We suggest that GAL10-ncRNA tunes the expression of GAL genes by additional mechanisms besides suppressing leaky transcription from the GAL1-10 promoter. PMID: 27073161
  2. NuA4 Histone Acetyltransferase associates with the GAL10 antisense transcription initiation site at the 3' end of the coding sequence. PMID: 26755557
  3. RNA polymerase II is targeted to the antisense initiation site at GAL10 by Reb1p activator as well as general transcription factors (e.g., TFIID, TFIIB, and Mediator) for antisense transcription initiation. PMID: 23836882
  4. molecular amalysis of galactose mutarotase/UDP-galactose 4-epimerase from Saccharomyces cerevisiae PMID: 15795221
  5. The epimerase and mutarotase activities do not appear to influence each other. Protein is a dimer in solution. PMID: 17253981
  6. The 3-dimensional structure of the dual-function enzyme Gal10p was determined at 1.85-Angstrom resolution. It folds into 2 separate and distinct units (corresponding to the epimerase and mutarotase domains) that are connected by a simple Type II turn. PMID: 15795221

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Protein Families
NAD(P)-dependent epimerase/dehydratase family; Aldose epimerase family
Database Links

KEGG: sce:YBR019C

STRING: 4932.YBR019C

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