GAS1 Antibody

Code CSB-PA324339XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAS1 Polyclonal antibody
Uniprot No.
Target Names
GAS1
Alternative Names
GAS1 antibody; GGP1 antibody; YMR307W antibody; YM9952.09 antibody; 1,3-beta-glucanosyltransferase GAS1 antibody; EC 2.4.1.- antibody; Glycolipid-anchored surface protein 1 antibody; Glycoprotein GP115 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) GAS1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis.
Gene References into Functions
  1. The results suggest that the dysfunction of Gas1 plays a positive role in the maintenance of rDNA integrity by decreasing PKA activity and inducing the accumulation of Msn2/4 in the nucleus. PMID: 24981510
  2. Gas1p functions in cell wall formation, and is the primary glucan-remodeling enzyme required in vegetative growth and during reentry into the proliferative state. PMID: 21389112
  3. Results suggest that Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar, and reveal a new mechanism of protein immobilization at specific sites of the cell envelope. PMID: 19793924
  4. Because the catalytic activity of Gas1 is required for telomeric silencing, Gas1 localizes to the nuclear periphery, and Gas1 and Sir2 physically interact. PMID: 19541632
Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor. Secreted, cell wall.
Protein Families
Glycosyl hydrolase 72 family
Database Links

KEGG: sce:YMR307W

STRING: 4932.YMR307W

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