Product Details

Uniprot No.

Target Names

GIF1

Alternative Names

GRF1-interacting factor 1 (AtGIF1) (Protein ANGUSTIFOLIA 3) (Transcription coactivator GIF1) GIF1 AN3 GIF At5g28640 F4I4.20

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana GIF1 protein (1-210aa)

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The generation of the GIF1 polyclonal antibody initiates with the use of a recombinant Arabidopsis thaliana GIF1 protein segment (encompassing amino acids 1-210) as the immunogen, which is administered to a rabbit to induce an antibody response. Serum drawn from the rabbit contains polyclonal antibodies, which are subsequently purified via affinity chromatography. This GIF1 antibody's functionality is established through ELISA and WB assays, affirming its proficiency in detecting Arabidopsis thaliana GIF1 protein in experimental settings.

Arabidopsis thaliana GIF1 protein is a transcription factor that plays a role in regulating plant growth and development. Specifically, it has been shown to be involved in the regulation of root elongation and gravitropism, which is the ability of plants to sense and respond to gravity. GIF1 also plays a role in the regulation of flowering time and seed germination.

Related Products

Target Background

Function

Transcription coactivator that plays a role in the regulation of cell expansion in leaf and cotyledons tissues. Component of a network formed by miR396, the GRFs and their interacting factors (GIFs) acting in the regulation of meristem function, at least partially through the control of cell proliferation. Appears to function synergistically with GRF1 as a transcriptional coactivator. Acts together with GRF5 for the development of appropriate leaf size and shape through the promotion and/or maintenance of cell proliferation activity in leaf primordia. Plays a role in adaxial/abaxial patterning and growth in leaf morphogenesis. GIFs are involved in the positive regulation of cell proliferation of lateral organs in a functionally redundant manner. Together with GATA18/HAN, mediates cotyledon identity by preventing ectopic root formation through the repression of PLT1 expression.

Gene References into Functions

GRF-GIF duo regulates the meristematic and pluripotent competence of carpel margin meristems. PMID: 29114079
Rice MKB3 and Arabidopsis AN3 have conserved functions and effects on leaf development PMID: 29567670
AN3 and YDA mutations both disrupt normal sucrose and glucose contents and cause altered seed size in an3 or yda mutants. PMID: 28489361
Nuclear localization of AN3 during initial leaf growth results in differential expression of important transcriptional regulators, including GROWTH REGULATING FACTORs (GRFs). PMID: 24443518
ANGUSTIFOLIA3 (AN3) moves into Arabidopsis epidermal cells after being synthesized within mesophyll cells and helps control epidermal cell proliferation. Interference with AN3 movement results in abnormal leaf size and shape. PMID: 23602479
AN3 and AtGRF5 act together and are required for the development of appropriate leaf size and shape through the promotion and/or maintenance of cell proliferation. [AN3] PMID: 15960617
Data found that an3-dependent compensation is a non-cell-autonomous process, and that an3 cells seem to generate and transmit an intercellular signal that enhances postmitotic cell expansion. PMID: 21068059
Overexpression of miR396 in a mutant lacking GRF-INTERACTING FACTOR 1 severely compromises the shoot meristem. PMID: 20023165
The GIF gene family plays important roles in the control of cell proliferation via cell cycle regulation and in other developmental properties that are associated with shoot apical meristem function. PMID: 19648231

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Protein Families

SS18 family

Tissue Specificity

Strongly expressed in actively growing and developing tissues, such as roots, upper stems, and shoot tips and flower buds. Also expressed in mature flowers. Not expressed in the shoot apical meristem (SAM). Highly accumulated in the proximal part of leaf

Database Links

KEGG: ath:AT5G28640

STRING: 3702.AT5G28640.1

UniGene: At.22564

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Code	CSB-PA781430XA01DOA
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: GIF1 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa
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GIF1 Antibody

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Target Background