glnA1 Antibody

Code CSB-PA142103XA01FSG
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Product Details

Full Product Name
Rabbit anti-Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) glnA1 Polyclonal antibody
Uniprot No.
Target Names
glnA1
Alternative Names
glnA1 antibody; glnA antibody; Rv2220 antibody; MTCY190.31 antibody; MTCY427.01Glutamine synthetase antibody; GS antibody; EC 6.3.1.2 antibody; Glutamate--ammonia ligase antibody; Glutamine synthetase I beta antibody; GSI beta antibody
Raised in
Rabbit
Species Reactivity
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Immunogen
Recombinant Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) glnA1 protein
Immunogen Species
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Also able to use GTP. D-glutamate is a poor substrate, and DL-glutamate shows about 50% of the standard specific activity. Also plays a key role in controlling the ammonia levels within infected host cells and so contributes to the pathogens capacity to inhibit phagosome acidification and phagosome-lysosome fusion. Involved in cell wall biosynthesis via the production of the major component poly-L-glutamine (PLG). PLG synthesis in the cell wall occurs only in nitrogen limiting conditions and on the contrary high nitrogen conditions inhibit PLG synthesis (Probable).
Gene References into Functions
  1. All resistant strains had a single-nucleotide deletion in the 5' region of glnA1. PMID: 21875360
  2. These results appropriately characterize glutamine synthetase of Mycobacterium tuberculosis exhibiting transacylase action as a moonlighting protein. PMID: 21411303
  3. fndings show that only glnA1 of the four glutamine synthetase genes is required for in vitro growth of M. tuberculosis and all four glutamine synthetase genes are transcribed but only GlnA1 is produced in microgram quantities in M. tuberculosis cultures PMID: 16262797
Subcellular Location
Cytoplasm.
Protein Families
Glutamine synthetase family
Database Links

KEGG: mtu:Rv2220

STRING: 83332.Rv2220

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