Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) gyrB Polyclonal antibody

Uniprot No.

P0AES6

Target Names

gyrB

Alternative Names

gyrB antibody; acrB antibody; cou antibody; himB antibody; hisU antibody; nalC antibody; parA antibody; pcbA antibody; b3699 antibody; JW5625DNA gyrase subunit B antibody; EC 5.6.2.2 antibody; Type IIA topoisomerase subunit GyrB antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) gyrB protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner to maintain chromosomes in an underwound state. This makes better substrates for topoisomerase 4 (ParC and ParE) which is the main enzyme that unlinks newly replicated chromosomes in E.coli. Gyrase catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes. Relaxes negatively supercoiled DNA in an ATP-independent manner. E.coli gyrase has higher supercoiling activity than other characterized bacterial gyrases; at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli. E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical. The enzymatic differences between E.coli gyrase and topoisomerase IV are largely due to the GyrA C-terminal domain (approximately residues 524-841) and specifically the GyrA-box.

Gene References into Functions

Among nalidixic acid resistant isolates...1 (2%) isolate showed gyrB (A660-T660) SNP. PMID: 27496516
The GyrB subunit from Escherichia coli has been investigated, namely the ATP binding pocket both considering the protein without ligands and bound with the inhibitors clorobiocin, novobiocin and 5'-adenylyl-beta-gamma-imidodiphosphate. PMID: 21216167
ParA binds DNA non-specifically in the presence of ATP. PMID: 20659294
The insert domain of gyrB may have two functions, acting as a steric buttress to pre-configure the primary DNA-binding site, and serving as a relay that may help coordinate communication between different functional domains. PMID: 20675723
double role played by metal ions in the catalytic steps involving DNA gyrase B PMID: 16223508
These results reveal an important function for Par systems in plasmid DNA segregation that is likely to be conserved in bacteria. PMID: 18208495

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Subcellular Location

Cytoplasm.

Protein Families

Type II topoisomerase family

Database Links

KEGG: ecj:JW5625

STRING: 316407.85676345

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gyrB Antibody

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