HAP2 Antibody

Code CSB-PA518991XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HAP2 Polyclonal antibody
Uniprot No.
Target Names
HAP2
Alternative Names
HAP2 antibody; GCS1 antibody; At4g11720 antibody; T5C23Protein HAPLESS 2 antibody; GENERATIVE CELL SPECIFIC 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HAP2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Required for male fertility. Plays a role in pollen tube guidance and successful gamete attachment. Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm. Mediates the fusion of cell membranes. Not required for pollen tube outgrowth.
Gene References into Functions
  1. no significant difference in ovule-targeting frequency between hap2-1 and HAP2 pollen tubes, indicating that the gcs1/hap2 mutation does not affect the ability of pollen tubes to target the ovule; results supply a clue that may allow conclusive settlement of the disagreement regarding the function of GCS1/HAP2 during the angiosperm sexual reproduction process PMID: 28791484
  2. These results provide data identifying the essential functional features of HAP2, a highly conserved sperm fusion protein. PMID: 20333238
Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein. Cell membrane; Single-pass type I membrane protein.
Protein Families
HAP2/GCS1 family
Tissue Specificity
Expressed only in mature pollen, in the two sperm cells.
Database Links

KEGG: ath:AT4G11720

STRING: 3702.AT4G11720.1

UniGene: At.54282

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